Veterinary Diagnostics

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Bovine Viral Diarrhea Virus (BVDV) / Border Disease (BD)

ID Gene™ BVD/BD Triplex

PCR

Real-time RT-qPCR for the qualitative detection of all serotypes of the BVD / BD viruses in ruminant blood, serum, milk, organs and ear notch samples (individual samples and pools)

The test amplifies a target sequence as well as endogenous and exogenous controls.
It includes a positive amplification control.
A standardized, freeze-dried positive extraction control to monitor variations in analytical sensitivity is sold separately (product code : PEC-BVD).
This qPCR may be used with direct lysis for ear notch samples (no extraction step required, product code : DLB)

Advantages

Specifications

Format

  • Accurate and reliable results
    • DLPCR < 10 copies / PCR
    • Includes internal controls to guarantee robust results:
      • an endogenous internal control, to ascertain presence and correct conservation of the sample
      • an exogenous internal control, to evaluate extraction efficiency and confirm the absence of inhibitors
    • Validated by the French national reference laboratory according to the French standard NFU47-600-2
  • Allows for optimized workflow
    • Rapid, 1 hour amplification protocol
    • Ready-to-use : simply dispatch the reaction mix, add the sample and run the test
  • Flexible and convenient
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate

Method :

Real-time RT-qPCR - Triplex - Qualitative

Species :

Ruminants

Sample types :

Blood, serum, ear notch samples, organs and milk

Product reference

Reactions

IDBVD-50 50
IDBVD-100 100

Associated products

Sample preparation
PCR

Single-tube system for the rapid preparation of DNA/RNA from various matrices. Read more

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more