Veterinary Diagnostics

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Lumpy Skin Disease Virus (LSDV) / Capripoxvirus (CPV)

ID Gene™ LSD DIVA Triplex

PCR

Real-time qPCR for the qualitative detection of a target DNA of LSDV, DNA of LSD based vaccine strains in whole blood, swabs and tissues

The test amplifies:

  • a target sequence in the DNA of LSDV
  • a target sequence in the DNA of LSDV-based vaccine strains
  • an exogenous internal control to be extracted along with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors in each well

Advantages

Specifications

Format

  • High performance: DLPCR< 10 copies / PCR
  • Rapid: 1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Reliable : validated by the EURL labs CODA-CERVA, Friedrich-Loeffler Institute and IZS dell’Abruzzoe del Molise

Method :

Real-time qPCR - Triplex - Qualitative

Species :

Ruminants

Sample types :

Whole blood, swabs, tissues

Product reference

Reactions

IDLSDIVA-50 50
IDLSDIVA-100 100

Associated products

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more