ID Gene™ Bluetongue genotype 12 Duplex

The ID Gene™ Bluetongue genotype 12 Duplex kit (product code: IDBTV12) is a ready-to-use, qualitative Duplex detection kit that simultaneously detects in real time:

a target sequence specific to the BTV12, conserved in all known BTV-12 strains in the FAM™ channel.
a sequence specific to the endogenous Non-Target Positive Control NTPCen, a ubiquitous gene inherently present in ruminant cells, in order to validate the integrity of the sample and the quality of the nucleic acid extraction, in the Cy5 channel.

Advantages

Specifications

Format

Specific detection and genotyping of BTV type 12 with a reliable RT-qPCR kit.

ACCURATE AND RELIABLE RESULTS

Exclusive detection of BTV-12 strains thanks to:
- Excellent exclusivity with respect to all other BTV and EHDV tested isolates
- Accurate detection of all BTV-12 tested strains

Very high diagnostic specificity and sensitivity
High robustness and stability ensuring consistency of results

CONVENIENT, EFFICIENT AND EASY TO USE

Optimized parallel testing for other pathogens (other BTV serotypes, EHDV, BVDV…):
- Same reagent volumes for all ID Gene™ BTV PCR kits to secure the analytical process
- Same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (BTV, BVDV, EHDV…) may be tested on the same plate

Time-saving solution: obtain results in less than 50 minutes with the rapid amplification protocol
Ready-to-use reagents

Method :

Real-time RT-PCR - Duplex - Qualitative

Species :

Ruminants

Sample types :

Whole blood (in EDTA tubes), spleen or organs from aborted foetuses (spleen, liver, heart)

Product reference

Reactions

IDBTV12-50 50

IDBTV12-100 100

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Associated products

Positive extraction control

Freeze-dried BTV1-12 Positive Extraction Control

PCR

Freeze-dried control consisting of an Inactivated BTV genotype 1 (BTV-1) positive whole blood sample and a nucleic acid BTV genotype 12 (BTV-12), in a virus-negative whole blood matrix. Read more

Extraction

ID Gene™ Mag Fast Extraction Kit

PCR

Veterinary Diagnostics

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Real-time RT-PCR kit for the qualitative detection of BTV-12 RNA. Suitable samples: whole blood (in EDTA tubes), spleen and organ from aborted fetuses (spleen, liver, heart).