Veterinary Diagnostics

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Bluetongue virus (BTV)

ID Gene™ Bluetongue genotype 3 Duplex

PCR

Real-time RT-PCR kit for the qualitative detection of Bluetongue virus genotype 3 RNA in whole blood (in EDTA tubes), spleen or organs from aborted foetuses.

The test amplifies a target sequence in the genome of the Bluetongue Virus of genotype 3 (BTV-3), responsible of Bluetongue disease.
A qualitative Duplex test, it simultaneously amplifies:

  • target RNA
  • an endogenous non-target positive control.

Advantages

Specifications

Format

ACCURATE AND RELIABLE RESULTS
  • Exclusive detection of BTV3 strains thanks to:
    • Excellent exclusivity with respect to all other BTV and EHDV tested isolates
    • Accurate detection of all BTV3 tested strains
  • Validated by the French National Reference Laboratory for BTV and EHDV (ANSES)
  • Very high diagnostic specificity and sensitivity
  • High robustness and stability ensuring consistency of results
CONVENIENT, EFFICIENT AND EASY TO USE
  • Optimized parallel testing for other pathogens (other BTV genotypes, EHDV, BVDV…):
    • Same reagent volumes for all ID Gene™ BTV PCR kits to secure the analytical process
    • Same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (BTV, BVDV, EHDV…) may          be tested on the same plate
  • Time-saving solution: obtain results in less than 50 minutes with the rapid amplification protocol
  • Ready-to-use reagents

Method :

Real-time RT-PCR - Duplex - Qualitative

Species :

Ruminants

Sample types :

Whole blood (in EDTA tubes), spleen or organs from aborted foetuses

Product reference

Reactions

IDBTV3-50 50
IDBTV3-100 100

Associated products

Positive extraction control
PCR

Lyophilised positive extraction control consisting of inactivated BTV genotype 8 (BTV-8) positive bovine whole blood, BTV8 nucleic acid, inactivated BTV genotype 3 (BTV-3) positive bovine whole blood, BTV3 nucleic acid, inactivated EHDV positive bovine whole blood, BTV4 nucleic acid and negative bovine blood spiked with inactivated BTV genotype 4 (BTV-4) vaccine strain. Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more