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Home   »   Products   »   Veterinary diagnostics   »  Ruminants   »   Epizootic Haemorrhagic Disease Virus (EHDV)   »   ID Gene™ EHDV Advantage Duplex
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Epizootic Haemorrhagic Disease Virus (EHDV)

ID Gene™ EHDV Advantage Duplex

PCR

Real-time RT-qPCR kit for the qualitative detection of EHDV RNA in whole blood (in EDTA tubes), spleen or organs from aborted animals

The ID Gene™ EHDV Advantage Duplex kit is a RT-qPCR kit that amplifies a target sequence in the genome of the EHDV (Epizootic Hemorrhagic Disease Virus), responsible of the Epizootic Hemorrhagic Disease.
This kit is a qualitative Duplex test. It simultaneously amplifies target RNA as well as an endogenous non-target positive control.

Advantages

Specifications

Format

SUPERIOR TEST PERFORMANCE

  • Excellent in silico and experimental inclusivity: detects all EHDV genotypes
  • No-cross reactions with Bluetongue viruses
  • High analytical sensitivity: earliest detection of EHDV among commercial kits (mean difference of ΔCq= 4 with respect to another commercially-available PCR)
  • Excellent diagnostic specificity and sensitivity
  • Best results in a comparative study led by the WOAH Reference Laboratory for BTV of Teramo-Italy
  • Test evaluated by the French National Reference Laboratory for BTV & EHDV (ANSES, Maisons-Alfort)
  • Stable reagents to ensure consistency of results over time

 

EFFICIENT, CONVENIENT AND EASY-TO-USE

  • Optimized parallel testing for several pathogens (specific BTV genotypes, EHDV, BVDV…):
    • Same reagent volumes as those of the ID Gene™ BTV PCR kits and ID Gene™ BTV & EHDV Advantage Triplex PCR kit, to secure the analytical process
    • Same amplification protocol common to all ID Gene™ PCR kits, meaning that several pathogens (BTV, BVDV, EHDV…) may be tested on the same plate
  • Ready-to-use reagents
  • Fast time to results: 50 minutes

Method :

RT-qPCR - Duplex - Qualitative

Species :

Ruminants (cattle, sheep, goats and cervids)

Sample types :

Whole blood (in EDTA tubes), spleen or organs from aborted fetuses

Product reference

Reactions

IDEHDVADV-50 50
IDEHDVADV-100 100
Contact your local rep

Associated products

Positive extraction control
Freeze-dried EHDV Positive Extraction Control
PCR

Freeze-dried control consisting of an inactivated strain of the Epizootic Haemorrhagic Disease Virus, diluted in negative bovine blood. Read more

Positive extraction control
Freeze-dried BTEHD Target Positive Control
PCR

Lyophilised positive extraction control consisting of inactivated BTV genotype 8 (BTV-8) positive bovine whole blood, BTV8 nucleic acid, inactivated BTV genotype 3 (BTV-3) positive bovine whole blood, BTV3 nucleic acid, inactivated EHDV positive bovine whole blood, BTV4 nucleic acid and negative bovine blood spiked with inactivated BTV genotype 4 (BTV-4) vaccine strain. Read more

Extraction
ID Gene™ Mag Fast Extraction Kit
PCR