Veterinary Diagnostics

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Influenza A

ID Gene Lyo™ Influenza A Multi-species Triplex

PCR

Real-time RT-PCR kit for the qualitative detection of Influenza A virus RNA on various samples (depending on species).

The ID Gene Lyo™ Influenza A Multi-species Triplex kit is a freeze dried, qualitative Triplex detection kit that simultaneously detects, in real time:

  • A target sequence specific of Influenza A virus in the FAM™ channel,
  • A sequence specific of the Endogenous Non-Target Positive Control (NTPCen), in the VIC®/HEX™ channel,
  • A sequence specific of the Exogenous Non-Target Positive Control (NTPC-EXO1), added to environmental samples and optionnaly to all sample prior to extraction, to assess the presence of RT-qPCR inhibitors, in the Cy5 Channel.

Advantages

Specifications

Format

Accurate and reliable detection across all susceptible species
  • Highly sensitive and specific detection of Influenza A virus across all susceptible avian and mammalian species, including poultry, swine, horse and cattle
  • The oligonucleotide designs are updated to ensure optimal detection of all circulating strains
Validated performance with high sensitivity and specificity
  • Extensively tested and validated across a wide range of matrices and animal species with 100% specificity (CI95% = 81,6%-100%, n=17) and 100% sensitivity (CI95% = 98,3%-100%, n=317)
  • Robust detection across various sample types, and depending on species:
    • Swabs: tracheal, oropharyngeal, cloacal, nasopharyngeal, nasal/deep nasal
    • Organs: lung, trachea, liver, spleen, kidney, pancreas, heart, intestine, brain
    • Environmental samples: boot swabs, surface wipes, drinking water swabs
    • Other matrices: feaces, feathers, milk, oral fluids, tracheobronchial wash, nucleic acid storage cards, viral culture
Convenient, efficient and flexible
  • Freeze-dried Amplification Reaction Mix (ARM):
    • For cost-effective shipping at room temperature
    • High stability in its freeze-dried format, and also after reconstitution
  • Blue ARM for better visibility and easier sample deposit

Method :

Real-time PCR – Triplex - Qualitative

Species :

Avian, Swine, Horse, Cattle and other susceptible species

Sample types :

AVIAN : Swab (tracheal, oropharyngeal, cloacal) individual or pool of 5; Organ (lung-trachea, liver-liver-kidney-pancreas-heart, intestine, brain) individual or pool of 5; Environmental samples by surface wipe, boot swab or drinking water swabs; individual; Faeces individual or pool of 5; Feather bulb, individual or pool of 5. SWINE: Nasal / nasopharyngeal swabs individual or pool of 5; Oral fluids individual or pool of 5 and oral fluids collected on a rope with a pen; Tracheobronchial wash individual or pool of 5; Organ individual or pool of 5; Environmental samples by surface wipe or boot swab; individual. HORSE: Nasal / nasopharyngeal swab in MEM or dry medium, individually. CATTLE: Milk individual or tank milk; Nasal/nasopharyngeal swab individual or pool of 5. OTHER: Viral culture, individual; Nucleic acid storage card, individual or pool of 5.

Product reference

Reactions

IDFLUAMSL-50 50
IDFLUAMSL-100 100

Associated products