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Home   »   Products   »   Veterinary diagnostics   »  Ruminants   »   Lumpy Skin Disease Virus (LSDV) / Capripoxvirus (CPV)   »   ID Gene Lyo™ LSD DIVA Triplex 2.0
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Lumpy Skin Disease Virus (LSDV) / Capripoxvirus (CPV)

ID Gene Lyo™ LSD DIVA Triplex 2.0

PCR

Real time PCR kit for the qualitative detection of Lumpy Skin Disease virus and LSDV based vaccine strains DNA.

The ID Gene Lyo™ LSD DIVA Triplex 2.0 (product code: IDLSDIVA2L) kit is a qualitative Triplex real time PCR kit that simultaneously detects:

  • A target sequence specific to the Lumpy skin Disease virus (LSDV) in the FAM™ channel.
  • A target sequence specific to the LSDV based vaccine strains, in the VIC®/ HEX™ channel.
  • A sequence specific to the Exogenous Non-Target Positive Control (NTPC-LSDIVA) in the Cy5 channel.

Advantages

Specifications

Format

Efficient freeze-dried PCR : detection and differentiation between LSDV wild type strains (including recombinant strains), LSDV-based vaccine strains, and an exogenous control.

The best tool for a DIVA strategy

  • Reliable differentiation between the Neethling response following vaccination, and field infection, thanks to the correct classification of clades 1.1, 1.2 and 2.5 as wild-type strains.
  • Upgraded PCR : efficient detection of clade 2.5 as a wild-type strain.
  • Secure the whole analytical process thanks to the use of an exogenous control to monitor all qPCR steps and to check for inhibitions
Convenient and easy-to-use
  • Freeze-dried reagents allowing for shipping at room temperature: easier, cost-effective and environmentally-friendly.
  • Obtain a DIVA result in a single PCR well.
  • Ready-to-use master mix after reconstitution
  • Time-saving solution: obtain results in less than 40 minutes with the rapid amplification protocol

Method :

Real-time RT-PCR – Triplex - Qualitative

Species :

Ruminants

Specimens :

Whole blood, swabs (oral and nasal), tissues (skin lesions /nodules)

Product reference

Reactions

IDLSDIVA2L-50 50
IDLSDIVA2L-100 100
Contact your local rep

Associated products

Extraction
ID Gene™ Mag Fast Extraction Kit
PCR
Positive extraction control
Freeze-dried LSDIVA Positive Extraction Control
PCR

Freeze-dried preparation of inactivated LSDV Neethling vaccine and inactivated LSDV field strain, diluted in a negative bovine whole blood matrix. Read more