Veterinary Diagnostics

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Epizootic Haemorrhagic Disease Virus (EHDV)

ID Screen® EHDV Competition

ELISA

Competitive ELISA for the detection of antibodies against the EHDV VP7 protein in sheep, goat, cattle, buffalo or deer serum and plasma samples

Advantages

Specifications

Format

References

Downloads

  • Detects all serotypes, as the VP7 protein is highly conserved
  • Early detection of seroversion (7-15 days post-infection)
  • The best combination of high Se and Spe
  • High exclusivity with respect to BTV
  • Results in only 90 minutes
  • Easier-to-use and better adapted to high throughput testing than the AGID test.
  • More convenient protocol than other commercial ELISAs: no sample pre-dilution step required and room temperature incubations.

Method :

Competitive ELISA

Species :

Domestic and wild ruminants

Specimens :

Serum and plasma

Coated antigen :

Recombinant VP7 protein

Conjugate :

Anti-VP7-HRP (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

EHDVC-5P 5 plates 480 12 x 8-well strips
EHDVC-10P 10 plates 960 12 x 8-well strips
PERFORMANCE EVALUATION
  1. Bréard E. et al. (2020). Evaluation of a commercial ELISA for detection of epizootic haem orrhagic disease antibodies in domestic and wild ruminant sera. Transbound Emerg Dis. 00:1–7.
EPIDEMIOLOGICAL STUDIES

CATTLE

  1. Anthonioz C. et al. (2025). Heterogenous within-herd seroprevalence against epizootic hemorrhagic disease virus type 8 (EHDV-8) after massive virus circulation in cattle in France, 2023. Frontiers in Veterinary Science, 12, 1562883.
  2. Chiuya T. et al. (2024). Exposure to arboviruses in cattle: Seroprevalence of Rift Valley fever, bluetongue, and epizootic hemorrhagic disease viruses and risk factors in Baringo County, Kenya. Pathogens, 13(8), 613.
  3. McVey D.S. et al. (2024). Evidence of active orbivirus transmission in 2016 in Kansas and Nebraska. Vector-Borne and Zoonotic Diseases, 24(6), 390-395.
  4. Bréard E. et al. (2023). Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021. Viruses, 15(6), 1259.
  5. Lorusso A. et al. (2023). Epizootic hemorrhagic disease virus serotype 8, Italy, 2022. Emerging Infectious Diseases, 29(5), 1063.
  6. Behar A. et al. (2022). Insights on Transmission, Spread, and Possible Endemization of Selected Arboviruses in Israel—Interim Results from Five-Year Surveillance. Veterinary Sciences, 9(2), 65.
  7. Sghaier S. et al. (2022). Epizootic haemorrhagic disease virus serotype 8 in Tunisia, 2021. Viruses, 15(1), 16.
  8. Yang H. et al. (2020). Novel serotype of epizootic hemorrhagic disease virus, China. Emerging Infectious Diseases, 26(12), 3081.

CATTLE AND SMALL RUMINANTS

  1. Ishaq M. et al. (2025). Serological evidence of epizootic hemorrhagic disease and serotypes of epizootic hemorrhagic disease virus in Pakistan. Acta Tropica, 107675.

WILDLIFE

  1. Lakatos I. et al. (2025). Cervids as a Promising Pillar of an Integrated Surveillance System for Emerging Infectious Diseases in Hungary: A Pilot Study. Animals, 15(13), 1948.
  2. Hidalgo-Hermoso E. et al. (2024). High Exposure to Livestock Pathogens in Southern Pudu (Pudu puda) from Chile. Animals 2024, 14, 526.
  3. Ruiz-Fons F. et al. (2024). Emergence of epizootic hemorrhagic disease in red deer (Cervus elaphus), Spain, 2022. Veterinary Microbiology, 292, 110069.
EXPERIMENTAL STUDIES
  1. Spedicato M. et al. (2024). Efficacy of an inactivated EHDV-8 vaccine in preventing viraemia and clinical signs in experimentally infected cattle. Virus Research, 347, 199416.
  2. Spedicato M. et al. (2023). Experimental infection of cattle, sheep, and goats with the newly emerged epizootic hemorrhagic disease virus serotype 8. Veterinaria Italiana, 59(4).
  3. Sailleau C. et al. (2019). Experimental infection of calves with seven serotypes of Epizootic Hemorrhagic Disease virus: production and characterization of reference sera. Veterinaria Italiana, 55 (4), 339-346.

Associated products

Internal reference material
ELISA

Freeze-dried positive bovine serum. Read more