Veterinary Diagnostics

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African Swine Fever Virus (ASFV)

ID Gene™ African Swine Fever Duplex

PCR

Real-time qPCR for the qualitative detection of a target sequence in the African Swine Fever (ASFV) viral genome in multiple sample types

The test amplifies

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

A  standardized, freeze-dried positive external control to monitor variations in analytical sensitivity is sold separately (product code : PEC-ASF).

Advantages

Specifications

Format

  • High performance: DLPCR 2.5 copies / PCR
  • Rapid: 1-hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing
  • Flexible: uses the same amplification protocol common to all ID Gene™ PCR tests: test other pathogens on the same plate
  • Practical: Proven stability allows for cost-effective shipping worldwide, without temperature control (no dry ice required)
  • Reliable: Validated by the EURL (CISA-INIA, Spain) and extensively used in ASF control programs around the world

Method :

qPCR - Duplex - Qualitative

Species :

Swine, wild pig or warthog

Sample types :

Serum, whole blood, oropharyngeal fluid or organ. Either individual samples or pools of up to 20 may be tested.

Product reference

Reactions

IDASF-50 50
IDASF-100 100

Associated products

Positive extraction control
PCR

Freeze-dried preparation of an inactivated ASFV strain diluted into a negative whole blood matrix. For use with the liquid format of the ID Gene™ African Swine Fever Duplex and Triplex kits. Read more

Extraction
PCR

The ID Gene™ Easy SPIN Extraction Kit – in 50 or 200 extractions format (product code: EZSPIN-50 or EZSPIN-200) – is a system of rapid extraction and purification of nucleic acids using silica columns, applicable to all matrices and veterinary pathogens. This kit is formulated without ethanol: wash buffers are supplied in concentrated solutions and […] Read more