Veterinary Diagnostics

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African Swine Fever Virus (ASFV)

ID Gene™ African Swine Fever Duplex

PCR

Real-time qPCR for the qualitative detection of a target sequence in the African Swine Fever (ASFV) viral genome in multiple sample types

The test amplifies

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

A  standardized, freeze-dried positive external control to monitor variations in analytical sensitivity is sold separately (product code : PEC-ASF).

Advantages

Specifications

Format

  • High performance: DLPCR 2.5 copies / PCR
  • Rapid: 1-hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing
  • Flexible: uses the same amplification protocol common to all ID Gene™ PCR tests: test other pathogens on the same plate
  • Practical: Proven stability allows for cost-effective shipping worldwide, without temperature control (no dry ice required)
  • Reliable: Validated by the EURL (CISA-INIA, Spain) and extensively used in ASF control programs around the world

Method :

qPCR - Duplex - Qualitative

Species :

Swine, wild pig or warthog

Sample types :

Serum, whole blood, oropharyngeal fluid or organ. Either individual samples or pools of up to 20 may be tested.

Product reference

Reactions

IDASF-50 50
IDASF-100 100

Associated products

Positive extraction control
PCR

Freeze-dried preparation of an inactivated ASFV strain diluted into a negative whole blood matrix. For use with the liquid format of the ID Gene™ African Swine Fever Duplex and Triplex kits. Read more

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more