Veterinary Diagnostics

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African Swine Fever Virus (ASFV)

ID Gene™ African Swine Fever Triplex

PCR

Real-time qPCR for the qualitative detection of a target sequence in African Swine Fever (ASFV) viral genome in multiple sample types from swine and wild boar, and for individual samples or pools of up to 20, thanks to high sensitivity

The test amplifies a target sequence as well as endogenous and exogenous internal controls.

Advantages

Specifications

Format

  • Accurate and reliable results
    • DLPCR < 5 copies / PCR
    • Internal controls ensure robust results:
      • the endogenous internal control ascertains sample presence and correct conservation
      • the exogenous internal control evaluates extraction efficiency and confirms the absence of inhibitors
    • Validated by the French national reference laboratory according to the French standard NFU47-600-2
  • Allows for optimized workflow
    • Ultrarapid results in only 35 minutes
    • Ready-to-use : simply dispatch the reaction mix, add the sample and run the test
  • Flexible and convenient
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate

Method :

Real-time qPCR - Triplex - Qualitative

Species :

Swine, wild pig or warthog

Sample types :

Serum, whole blood, oropharyngeal fluid, oropharyngeal swabs, organ and tissue (spleen, lymph node, tonsil, bone marrow and kidney). Either individual samples or pools of generally up to 20 may be tested

Product reference

Reactions

IDASFTRI-50 50
IDASFTRI-100 100

Associated products

Positive extraction control
PCR

Freeze-dried preparation of an inactivated ASFV strain diluted into a negative whole blood matrix. For use with the liquid format of the ID Gene™ African Swine Fever Duplex and Triplex kits. Read more

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more