Veterinary Diagnostics

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Bluetongue virus (BTV)

ID Gene™ Bluetongue Duplex

PCR

Real-time RT-qPCR for the qualitative detection of all serotypes of the Bluetongue virus in ruminant blood samples

The test amplifies:

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

The only commercial BTV PCR which includes a target positive control*:

  • to be extracted along with field samples in order to evaluate extraction efficiency;
  • calibrated at between 10 and 100 times the method detection limit in order to monitor variations in analytical sensitivity.

*The target positive control consists of inactiviated BTV, diluted in a virus-negative whole blood matricx, freeze-dried and calibrated at between 10 and 100 times the detection limit of the method (MDL).

Advantages

Specifications

Format

  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Rapid: 1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2.

Method :

Real-time RT PCR - Duplex - Qualitative

Species :

Ruminants

Sample types :

Blood

Product reference

Reactions

IDBTV-50 50
IDBTV-100 100

Associated products

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more