Veterinary Diagnostics

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Bluetongue virus (BTV)

ID Gene™ Bluetongue genotypes 8 and 4 Triplex 2.0

PCR

Real-time RT-PCR kit for the qualitative detection of BTV genotypes 8 and 4 RNA in whole blood (in EDTA tubes), spleen or organs from aborted animals.

The ID Gene™ Bletongue genotypes 8 and 4 Triplex test simultaneously detects in real time:

  • a target sequence specific of BTV-8, conserved in all known BTV-8 strains
  • a target sequence specific of BTV-4, conserved in all known BTV-4 strains
  • a sequence specific of a ubiquitous gene, inherently present in ruminant cells (NTPCen), in order to validate the integrity of the sample and the quality of the nucleic acid extraction

Advantages

Specifications

Format

ACCURATE AND RELIABLE RESULTS
  • Exclusive detection of BTV8 and BTV4 strains thanks to:
    • Accurate detection of all BTV8 and BTV4 tested strains, including the new BTV8 strains which emerged in France in 2023
    • Excellent exclusivity with respect to all other BTV and EHDV tested isolates
  • Validated by the French National Reference Laboratory for BTV and EHDV (ANSES)
  • Very high diagnostic specificity and sensitivity
  • High robustness and stability ensuring consistency of results
CONVENIENT, EFFICIENT AND EASY TO USE
  • Optimized parallel testing for other pathogens (other BTV serotypes, EHDV, BVDV…):
    • Same reagent volumes for all ID Gene™ BTV PCR kits to secure the analytical process
    • Same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (BTV, BVDV, EHDV…) may be    tested on the same plate
  • Time-saving solution: obtain results in less than 50 minutes with the rapid amplification protocol
  • Ready-to-use reagents

Method :

Real-time RT-PCR - Triplex - Qualitative

Species :

Ruminants

Sample types :

Whole blood (in EDTA tubes), spleen or organs from aborted animals

Product reference

Reactions

IDBTV8-4V2-50 50
IDBTV8-4V2-100 100

Associated products

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more