Veterinary Diagnostics

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Brucella abortus / B. melitensis / B. suis

ID Gene™ Brucella spp triplex

PCR

Real-time qPCR for the qualitative detection of Brucella spp genome in ruminant swabs (placental, vaginal, and cervical), milks, organs and tissues.

The test amplifies:

  • a target sequence to detect all Brucella species
  • an endogenous internal control, to ascertain presence and correct conservation of the sample
  • an exogenous internal control to be extracted along with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors in each well.

Advantages

Specifications

Format

  • High performance
    • DLPCR 12.5 copies / PCR
  • Rapid and easy-to-use
    • 1 hour amplification protocol
    • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate

Method :

Real time qPCR - Triplex - Qualitative

Species :

Ruminants

Sample types :

Swabs (placental, vaginal, and cervical), milks, organs and tissues

Product reference

Reactions

IDBRU-50 50
IDBRU-100 100

Associated products

Positive extraction control
PCR

Freeze-dried control consisting of inactivated Brucella ovis strain, diluted into a ruminant endo-cervical swab supernatant matrix. Read more

Reagent
PCR
Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more