ID Gene™ BTV & EHDV Advantage Triplex

This ready-to-use, qualitative Triplex detection kit simultaneously detects in real time:

A target sequence specific to the EHDV, conserved in all known serotypes from EHDV-1 to EHDV-8, including in the EHDV-8 strain recently isolated in Tunisia, Italy and Spain (serotype 8, Tun-2021), in the FAM™ channel.
A target sequence specific to the BTV, conserved in all known genotypes, including in the atypical BTV-25 strains and the strains recently isolated in Europe (BTV-3 NL-2023, BTV-8 FR-2023 and and BTV-4 Corse 2023), in the VIC®/ HEX™ channel.
A sequence specific to a ubiquitous gene, inherently present in ruminant cells (NTPCen), in order to validate the integrity of the sample and the quality of the nucleic acid extraction, in the Cy5 channel.

Advantages

Specifications

Format

ACCURATE AND RELIABLE RESULTS

Efficient differential diagnosis of Bluetongue (BTV) and Epizootic hemorraghic disease (EHDV) in a single well:

- Accurate detection of all BTV and EHDV tested strains, including BTV-8 and EHDV-8 strains detected in Europe in 2023
- Excellent inclusivity of all BTV and EHDV tested genotypes
- Efficient detection of co-infections

Validated by the French National Reference Laboratory for BTV & EHDV (ANSES)
Excellent diagnostic performance
High robustness and stability ensuring consistency of results

CONVENIENT, EFFICIENT AND FLEXIBLE

Optimized parallel testing for several pathogens (specific BTV serotypes, EHDV, BVDV…):
- Same reagent volumes as those of the ID GeneTM BTV PCR kits and ID GeneTM EHDV Advantage Duplex PCR kit, to secure the analytical process
- Same amplification protocol common to all ID Gene™ PCR kits, meaning that several pathogens (BTV, BVDV, EHDV…) may be tested on the same plate
Time-saving protocol: obtain results in less than 50 minutes with the rapid amplification protocol
Ready-to-use reagents

Method :

Real-time RT-PCR - Triplex - Qualitative

Species :

Ruminants (cattle, sheep, goats and cervids)

Sample types :

Whole blood (in EDTA tubes), spleen, organs from aborted fetuses (spleen, liver, heart)

Product reference

Reactions

IDBTEHDADV-50 50

IDBTEHDADV-100 100

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Associated products

Positive extraction control

EHDV Positive Extraction Control

PCR

Freeze-dried control consisting of an inactivated strain of the Epizootic Haemorrhagic Disease Virus, diluted in negative bovine blood. Read more

Positive extraction control

Freeze-dried BTV Target Positive Control

PCR

Freeze-dried positive extraction control consisting of an inactivated BTV-8 positive bovine whole blood and a negative bovine whole blood spiked with an inactivated BTV-4 vaccine strain. Read more

Extraction

ID Gene™ Mag Fast Extraction Kit

PCR

Veterinary Diagnostics

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Real-time RT-PCR kit for the qualitative detection of BTV and EHDV RNA in whole blood (in EDTA tubes), spleen or organs from aborted fetuses