Veterinary Diagnostics

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Infectious Bronchitis Virus (IBV)

ID Gene™ Infectious Bronchitis Duplex

PCR

RT-qPCR for the qualitative detection of avian coronaviruses (including all IBV variants and coronaviruses isolated from turkeys, pheasants and pigeons) in swabs, organs, or nucleic acid storage cards

The test amplifies a target sequence as well as an exogenous control.
The kit includes a positive amplification control (PAC).
A standardized, freeze-dried positive extraction control to monitor variations in analytical sensitivity is sold separately (product code : PEC-IBV).

Advantages

Specifications

Format

Downloads

  • Accurate and reliable results
    • DLPCR < 12.5 copies / PCR
    • Includes an exogenous internal control, extracted with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors
    • Detects avian coronaviruses, including all variants of all IBV strains (02, 4/91, Arkansas, D1466 QX, D181, IB80, It02, Massachusetts, Q1, QX…) and coronaviruses isolated from turkeys, pheasants and pigeons
  • Allows for optimized workflow
    • Rapid, 1 hour amplification protocol
    • Ready-to-use: simply dispatch the reaction mix, add the sample and run the test
  • Flexible and convenient
    • The kit is also validated on nucleic acid storage cards
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate

Method :

RT-qPCR - Duplex - Qualitative

Species :

Chickens, turkeys, pheasants, pigeons

Sample types :

Tracheal, oropharyngeal and cloacal swabs, organs (trachea, lung, kidney, caecal tonsils) or nucleic acid storage cards. Individual samples or pools up to 5.

Shipping temperature :

≤-16°C

Storage temperature :

-16°C; -26°C

Product reference

Reactions

IDIBV-50 50
IDIBV-100 100

Associated products

Positive extraction control
PCR

Freeze-dried preparation of an inactivated Infectious Bronchitis virus strain diluted into tracheal and oropharyngal swab supernatants from negative birds. Read more

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more