Veterinary Diagnostics

To place an order, please choose your country

Subscribe newsletter

Avian Mycoplasmoses

ID Gene™ MG/MS Triplex

PCR

RT-qPCR for the qualitative detection and differentiation of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in tracheal and oropharyngeal swabs or nucleic acid storage cards

The test amplifies two target sequences (Mycoplasma synoviae and Mycoplasma gallisepticum) as well as an endogenous internal control.
The positive control allows to validate the efficiency of the nucleic acid extraction and the RT-qPCR amplification processes and to monitor variations in analytical sensitivity.

Advantages

Specifications

Format

Downloads

  • Accurate and reliable results
    • DLPCR 10 copies/PCR for MG and 6.25 copies/PCR for MS
    • Includes an endogenous internal control, to ascertain presence and correct conservation of the sample
    • Excellent exclusivity: no cross-reactions observed (over 30 strains tested in an external study; results available upon request)
    • High inclusivity: detects all MG and MS field and vaccine strains
  • Allows for optimized workflow
    • Rapid, 1 hour amplification protocol
    • Ready-to-use: simply dispatch the reaction mix, add the sample and run the test
  • Flexible and convenient
    • The kit is also validated on nucleic acid storage cards
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
    • Freeze-dried format available: ambient temperature shipping and 24-month shelf-life

Method :

RT-qPCR - Triplex - Qualitative

Species :

Avian

Sample types :

Tracheal swabs, oropharyngeal swabs, or nucleic acid storage cards

Shipping temperature :

≤-16°C for the liquid version. Room temperature for the freeze-dried version.

Storage temperature :

-16°C; -26°C

Product reference

Reactions

IDMGMS-50 50
IDMGMS-100 100
IDMGMS-50FD (freeze-dried format) 50
IDMGMS-100FD (freeze-dried format) 100

Associated products

Positive extraction control
PCR

Freeze dried preparation of inactivated Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) bacteria diluted into tracheal and oropharyngeal swabs supernatants from negative birds. Read more

Sample preparation
PCR

Single-tube system for the rapid preparation of DNA/RNA from various matrices. Read more

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more