Veterinary Diagnostics

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Mycobacterium bovis complex / Tuberculosis

ID Gene™ Mycobacterium tuberculosis complex Duplex

PCR

Real-time qPCR for the qualitative detection of a sequence in the genome of members of the Mycobacterium tuberculosis complex (MTC) in lymph nodes and peripheral tissues

The test amplifies:

  • a target sequence from member(s) of the MTC (M. tuberculosis, M. bovis, M.africanum, M. microti, M.canetti, M.caprae, and M.pinnipedii)
  • an exogenous internal control to be extracted along with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors in each well

Advantages

Specifications

Format

  • High performance: (DLPCR = <10 copies / PCR and LDmethod = 300 UG/mL)
  • Rapid: <1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Developed according to the requirements of Terrestrial Animal Health Code (OIE) and the French National Reference Laboratory for Tuberculosis (ANSES)

Method :

Real-time qPCR - Duplex - Qualitative

Species :

Cattle, sheep, goats, deers, suids and badgers

Sample types :

Lymph nodes and peripheral tissues

Product reference

Reactions

IDTUB-50 50
IDTUB-100 100

Associated products

Positive extraction control
PCR

Freeze-dried preparation of inactivated Mycobacterium bovis BCG-Copenhagen vaccine strain (ATCC-27290) diluted in a negative lymph node supernatant. Read more

Extraction
PCR

The ID Gene™ Easy SPIN Extraction Kit – in 50 or 200 extractions format (product code: EZSPIN-50 or EZSPIN-200) – is a system of rapid extraction and purification of nucleic acids using silica columns, applicable to all matrices and veterinary pathogens. This kit is formulated without ethanol: wash buffers are supplied in concentrated solutions and […] Read more