Veterinary Diagnostics

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Neospora caninum

ID Gene™ Neospora Caninum Duplex

PCR

Real-time qPCR for the qualitative detection of Neospora caninum in brain, placenta and muscle samples

The test amplifies:

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

Advantages

Specifications

Format

  • High performance: DLPCR< 10 copies / PCR and specificity of 100%
  • Rapid: 1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Reliable :
    • validated by the French national reference laboratory and according to the French standard NFU47-600-
    • the only commercial N. caninum PCR to include a positive target control* to be extracted along with field samples in order to evaluate extraction, purification and amplification efficiency

* Target Positive Control (TPC) included in the kit: negative brain tissue spiked with inactivated N. caninum strain, and calibrated at between 10 and 100 times the detection limit of the method (MDL).

Method :

Real-time qPCR - Duplex - Qualitative

Species :

Ruminants

Sample types :

Brain, placenta, muscle

Product reference

Reactions

IDNEO-50 50
IDNEO-100 100

Associated products

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Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more