Veterinary Diagnostics

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Newcastle Disease Virus (NDV)

ID Gene™ Newcastle Disease Duplex

PCR

RT-qPCR for the qualitative detection of NDV in swabs, organs or nucleic acid storage cards (individual samples or pools up to 5)

The test amplifies a target sequence as well as an exogenous control.
The kit includes a positive amplification control (PAC).
A standardized, freeze-dried positive extraction control to monitor variations in analytical sensitivity is sold separately (product code : PEC-NDV).

Advantages

Specifications

Format

Downloads

  • Accurate and reliable results
    • DLPCR < 10 copies / PCR
    • Includes an exogenous internal control, extracted with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors
    • Detects all NDV strains (lentogenic, mesogenic, velogenic and vaccine strains)
  • Allows for optimized workflow
    • Rapid, 1 hour amplification protocol
    • Ready-to-use: simply dispatch the reaction mix, add the sample and run the test
  • Flexible and convenient
    • The kit is also validated on nucleic acid storage cards
    • Uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate

Method :

RT-qPCR - Duplex - Qualitative

Species :

Avian

Sample types :

Tracheal, oropharyngeal and cloacal swabs, organs (trachea, lung, spleen, liver, brain and cecal tonsil) or nucleic acid storage cards. Individual samples or pools up to 5.

Shipping temperature :

≤-16°C

Storage temperature :

-16°C; -26°C

Product reference

Reactions

IDNDV-50 50
IDNDV-100 100

Associated products

Positive extraction control
PCR

Freeze dried preparation of an inactivated Newcastle disease virus strain diluted into tracheal and oropharyngeal swab supernatants from negative birds. Read more