Veterinary Diagnostics

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Peste des Petits Ruminants Virus (PPRV)

ID Gene™ Peste des Petits Ruminants Duplex

PCR

Real-time RT-qPCR for the qualitative detection of a sequence in Peste des Petits Ruminants (PPRV) viral genome in whole blood, swabs (oral, ocular, nasal and rectal) organ or tissue

The test amplifies:

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

Advantages

Specifications

Format

RT-qPCR with field-proven efficiency to detect Peste des petits ruminants virus (PPRV) and an endogenous control.

Accurate and reliable results
  • Co-developped with CIRAD, a reference laboratory for PPR
  • Excellent performance:
    • Specificity and sensitivity: 100%
    • Highly specific detection of the four lineages of PPRV
  • Includes an endogenous control to certify sample presence and validate the extraction step
Rapid and convenient
  • Time-saving thanks to a streamlined solution :
    • Results in 80 minutes (extraction + amplification)
    • Ready-to-use reagents: dispatch the reaction mix, add the sample and run the test
  • Optimized testing process: the amplification protocol is common to all ID Gene kits : test the same sample for several pathogens on a single plate.
  • Availability of a positive extraction control* to validate all RT-qPCR steps from extraction to amplification.

*Sold separately

Method :

Real-time RT-qPCR - Duplex - Qualitative

Species :

Caprine and ovine

Sample types :

Whole blood, swabs (oral, ocular,nasal and rectal) organ or tissue

Product reference

Reactions

IDPPR-50 50
IDPPR-100 100

Associated products

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more

Positive extraction control
PCR

Freeze-dried positive extraction control consisting of inactivated PPR vaccine, diluted in virus-free whole blood, freeze-dried and calibrated at between 1 and 10 times the detection limit of the method. Read more