Veterinary Diagnostics

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Coxiella burnetii / Q fever

ID Gene™ Q Fever Triplex

PCR

Real-time qPCR for the qualitative or quantitative detection of Coxiella burnetii in ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples

The test amplifies:

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample
  • an exogenous internal control to be extracted along with field samples, to evaluate extraction efficiency and confirm the absence of inhibitors in each well.

Advantages

Specifications

Format

  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Rapid: 1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Validated by the OIE reference laboratory and according to the French standard NFU47-600-2 (underway)

Method :

Real-time qPCR - Triplex - Qualitative - Quantitative

Species :

Ruminants

Sample types :

Ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples

Product reference

Reactions

IDQF-50 50
IDQF-100 100

Associated products

Extraction
PCR

Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more