Veterinary Diagnostics

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Schmallenberg Virus (SBV)

ID Gene™ Schmallenberg Duplex

PCR

Real-time RT-qPCR for the qualitative detection of the Schmallenberg virus in ruminant blood, serum and organ samples

The test amplifies:

  • a target sequence
  • an endogenous internal control, to ascertain presence and correct conservation of the sample

It is the only commercial SBV PCR to include a positive target control to be extracted along with field samples in order to evaluate extraction efficiency and confirm the absence of inhibitors in each well. This control is cablirated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity. It consists of inactivated SBV, diluted in a virus-negative serum matrix, freeze-dried and calibrated at between 10 and 100 times the detection limit of the method (MDL).

Advantages

Specifications

Format

  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Rapid: 1 hour amplification protocol
  • Ready-to-use: no-need for reagent pre-mixing – simply dispatch the reaction mix, add the sample and run the test
  • Flexible : uses the same amplification protocol common to all ID Gene™ PCR tests, meaning that other pathogens (DNA or RNA) may be tested on the same plate
  • Reliable: Validated using the German FLI and the French national reference laboratory panels, and according to the French standard NFU47-600-2.
  • Detects all SBV serotypes

Method :

Real-time RT-qPCR - Duplex - Qualitative

Species :

Ruminants

Sample types :

Blood, serum, brain

Product reference

Reactions

IDSBV-50 50
IDSBV-100 100

Associated products

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Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). Read more

Extraction
PCR

Magnetic bead nucleic acid extraction system. Compatible with most open extraction robots for magnetic beads. May be used with most commercial amplification kits which are validated with this type of extraction protocol. Read more