The test amplifies:
- a target sequence
- an endogenous internal control, to ascertain presence and correct conservation of the sample
It is the only commercial SBV PCR to include a positive target control to be extracted along with field samples in order to evaluate extraction efficiency and confirm the absence of inhibitors in each well. This control is cablirated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity. It consists of inactivated SBV, diluted in a virus-negative serum matrix, freeze-dried and calibrated at between 10 and 100 times the detection limit of the method (MDL).