Veterinary Diagnostics

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Akabane virus (AKAV)

ID Screen® Akabane Competition

ELISA

Competitive ELISA for the detection of antibodies against AKAV in ruminant serum or plasma

Advantages

Specifications

Format

References

Downloads

  • High correlation with the virus neutralisation test (VNT)
  • Sensitive and specific, with clear separation of positive and negative results
  • No cross-reactions with SBV (see ref. 2 in the list of references)
  • Fast and convenient:
    • Ready-to-use components, with results in just over 2 hours
    • Easily check that all samples have been correctly deposited thanks to the dilution buffer which changes colour upon addition of the sera
  • Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT

Method :

Competitive ELISA

Species :

Ruminants

Specimens :

Serum and plasma

Coated antigen :

Purified virus

Conjugate :

Anti-G1-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

AKAC-4P 4 plates 384 12 x 8-well strips
AKAC-10P 10 plates 960 12 x 8-well strips
PERFORMANCE EVALUATION
  1. Li X. et al. (2019). Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum. BMC Veterinary Research, 15, 1-6.
  2. Kittelberger R. et al. (2013). Evaluation of two commercial enzyme-linked immunosorbent assay kits for the detection of serum antibodies against Akabane virus in cattle. Journal of Veterinary Diagnostic Investigation, 25(5), 645-648.
EPIDEMIOLOGICAL STUDIES

CATTLE

  1. Kim J. et al. (2024). Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014–2020. Journal of veterinary diagnostic investigation, 36(3), 428-437.
  2. Davoudi Y. et al. (2024). An outbreak of Akabane disease in a cattle herd on the Mughan plain, Iran. In Veterinary Research Forum (Vol. 15, No. 6, p. 303).
  3. Metwally S. et al. (2023). The First Serological Detection and Risk Factors Analysis of Akabane Virus in Egyptian Cattle. Animals, 13(11), 1849.
  4. Yildirim Y. et al. (2015). Molecular and Serological Investigation of Akabane Virus Infection in Cattle in Kars-Turkey. Israel Journal of Veterinary Medicine, 70(3).
  5. Oem J.K. et al. (2014). Serological characteristics of affected cattle during an outbreak of bovine enzootic encephalomyelitis caused by Akabane virus. Tropical animal health and production, 46, 261-263.
  6. Elhassan A.M. et al. (2014). A serological survey of Akabane virus infection in cattle in Sudan. International Scholarly Research Notices, 2014(1), 123904.

SMALL RUMINANTS

  1. Selim A. et al. (2025). Factors influencing Akabane virus seroprevalence in sheep and goats in Egypt’s Nile Delta. Tropical Animal Health and Production, 57(3), 1-7.
  2. Manavian M. et al. (2023). Seroprevalence and Risk Factors Associated with Akabane Virus Infection in Sheep and Goats in Fars Province, Iran. Archives of Razi Institute, 78(6), 1771.
  3. Hasircioglu S. et al. (2023). Searching Akabane and Pestivirus Infections in Native Breed Sheep with Abortion History. Agricultural Science Digest, 43(6), 852-857.
  4. Özsoy M. et al. (2021). Seroepidemiology of Akabane Virus Infection in Honamlı Goat Breed. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 9(3), 53-61.

CATTLE AND SMALL RUMINANTS

  1. Oluwayelu D.O. et al. (2016). Serological investigation of Akabane virus infection in cattle and sheep in Nigeria. Advances in virology, 2016(1), 2936082.
  2. Kojouri G.A. et al. (2015). Serological and molecular detection of Akabane virus in Iran. Iranian Journal of Applied Animal Science  5(3), 737-740.

ANTELOPES

  1. Orynbayev M.B. et al. (2016). Seroprevalence of infectious diseases in saiga antelope (Saiga tatarica tatarica) in Kazakhstan 2012–2014. Preventive veterinary medicine, 127, 100-104.
EXPERIMENTAL STUDIES
  1. Jeong H. et al. (2017). Experimental infection of goats with a newly isolated strain of Akabane virus that causes encephalomyelitis. Journal of Comparative Pathology, 157(2-3), 220-229.
  2. Lee H. et al. (2016). Experimental infection of cows with newly isolated Akabane virus strain (AKAV-7) causing encephalomyelitis. Veterinary Research, 47, 1-12.

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