Competitive ELISA for the detection of antibodies against AKAV in ruminant serum or plasma
Advantages
Specifications
Format
References
High correlation with the virus neutralisation test (VNT)
Sensitive and specific, with clear separation of positive and negative results
No cross-reactions with SBV (see ref. 2 in the list of references)
Fast and convenient:
Ready-to-use components, with results in just over 2 hours
Easily check that all samples have been correctly deposited thanks to the dilution buffer which changes colour upon addition of the sera
Cost-effectivefor the analysis of large numbers of samples, notably in comparison with VNT
Method :
Competitive ELISA
Species :
Ruminants
Specimens :
Serum and plasma
Coated antigen :
Purified virus
Conjugate :
Anti-G1-HRP conjugate (concentrated 10X)
Product reference
Kit format
Reactions
Plate format
AKAC-4P4 plates38412 x 8-well strips
AKAC-10P10 plates96012 x 8-well strips
Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia.
Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the 2013 Epizone Congress, Brussels, Belgium.