Veterinary Diagnostics

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Akabane virus (AKAV)

ID Screen® Akabane Competition

ELISA

Competitive ELISA for the detection of antibodies against AKAV in ruminant serum or plasma

Advantages

Specifications

Format

References

  • High correlation with the virus neutralisation test (VNT)
  • Sensitive and specific, with clear separation of positive and negative results
  • No cross-reactions with SBV (see ref. 2 in the list of references)
  • Fast and convenient:
    • Ready-to-use components, with results in just over 2 hours
    • Easily check that all samples have been correctly deposited thanks to the dilution buffer which changes colour upon addition of the sera
  • Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT

Method :

Competitive ELISA

Species :

Ruminants

Specimens :

Serum and plasma

Coated antigen :

Purified virus

Conjugate :

Anti-G1-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

AKAC-4P 4 plates 384 12 x 8-well strips
AKAC-10P 10 plates 960 12 x 8-well strips
  1. Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia.
  2. Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the 2013 Epizone Congress, Brussels, Belgium.

Associated products

Internal reference material
ELISA

Positive freeze-dried ovine serum. Read more

Internal reference material
ELISA

Negative freeze-dried bovine serum. Read more