ID Screen® Brucellosis Milk Indirect

The assessment of the diagnostic performance of this ELISA kit on field samples has shown that positive bovine milk samples may be detected in pools of up to 250 samples.

* Note: The analysis of milk from small ruminants and the analysis of individual milks are not official EU tests.

Advantages

Specifications

Format

References

Downloads

Calibrated according to the Annex C European directive CEE 64/432 and OIE requirements
Straightforward test for individual or pooled milks

Method :

Indirect ELISA

Species :

Ruminants

Specimens :

Bovine (cattle and buffalo), ovine and caprine milk (individual or up to 250 pooled samples).

Coated antigen :

Purified Brucella LPS

Conjugate :

Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

BRUMILK-5P 5 plates 480 12 x 8-well strips

BRUMILK-10P 10 plates 960 12 x 8-well strips

Almashhadany D.A (2021). Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques. Iraqi Journal of Veterinary Sciences, 35 (4), 663-668.
Nawaz M. et al. (2020). Bovine and caprine brucellosis detected by milk indirect ELISA and milk ring test in Islamabad Capital Territory, Pakistan. Pakistan Journal of Zoology, 53(1), 391-394.
Khan T.I. et al. (2018). Milk indirect-ELISA and milk ring test for screening of brucellosis in buffaloes, goats and bulk tank milk samples collected from two districts of Punjab, Pakistan. Pak Vet J, 38(1): 105-108.
Hatem A.A. (2017). The prevalence of brucellosis of farm animals using serum-and milk-ELISA test in Al-Najaf province. Al-Kufa University Journal for Biology, 9(2), 9-14.
Kamwine M. et al. (2017). Prevalence of antibodies to Brucella species in commercial raw bovine milk in Southwestern Uganda. BMC Research Notes, 10, 1-5.
Beauvais W. et al. (2016). Empirical Bayes estimation of farm prevalence adjusting for multistage sampling and uncertainty in test performance: a Brucella cross-sectional serostudy in southern Kazakhstan. Epidemiology & Infection, 144(16), 3531-3539.

External references_BRUMILK_VER0125_ website_doc1456 228.70 KB

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Associated products

Internal reference material

Freeze-dried negative bovine milk

ELISA

Freeze-dried bovine milk. Read more

Veterinary Diagnostics

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Indirect ELISA for the detection of antibodies against Brucella abortus or melitensis in bovine, ovine and caprine milk. The test may be used on individual or bulk milks*.