Double Antigen ELISA for the detection of antibodies against capripoxviruses including lumpy skin disease virus (LSDV), sheeppox virus (SPPV) and goatpox virus (GTPV) in serum or plasma from cattle, sheep, goats or other susceptible species.
First commercially-available ELISA which allows for the detection of Lumpy Skin Disease antibodies
Very high specificity in CPV-free regions (>99.7%). Should not cross-react with parapox viruses
Detects antibodies against LSDV either in vaccinated or infected animals. At least equivalent sensitivity compared to IPMA and improved sensitivity compared to VNT (detection of antibodies as of 20 dpv up until at least 7 months post-vaccination)
Easy to handle, with ready-to-use reagents, and allows for high throughput screening without requiring high level containment facilities
Double Antigen ELISA
Ruminants and other susceptible species
Serum and plasma
Coated antigen :
CPV purified antigen
CPV purified antigen-HRP (concentrated 10X)
CPVDA-2P2 plates19212 x 8-well strips
CPVDA-5P5 plates48012 x 8-well strips
Haegeman A. et al. (2023). Duration of Immunity Induced after Vaccination of Cattle with a Live Attenuated or Inactivate Lumpy Skin Disease Virus Vaccine. Microorganisms 11, 210.
Hakobyan V. et al. (2023). The Serological Response in Cattle following Administration of a Heterologous Sheep Pox Virus Strain Vaccine for Protection from Lumpy Skin Disease; Current Situation in Armenia. Veterinary Sciences, 10(2), 102.
Suwankitwat N. et al. (2023). Long-term monitoring of immune response to recombinant lumpy skin disease virus in dairy cattle from small-household farms in western Thailand. https://doi.org/10.21203/rs.3.rs-2534351/v1.
Fay P. C. et al. (2022). The immune response to lumpy skin disease virus in cattle is influenced by inoculation route. Frontiers in Immunology, 13, 6947.
Hussien M. O. et al. (2022). Serological, virological and molecular diagnosis of an outbreak of lumpy skin disease among cattle in Butana area, Eastern Sudan. Veterinary Medicine and Science, 1–7.
Ibrahim A.I. et al. (2022). Serodiagnosis of Lumpy Skin Disease Using Sheep Pox Virus Compared to a Commercial ELISA Kit. Journal of Applied Veterinary Sciences, 7(1), pp. 46-52.
Ko Y. S. et al. (2022). Serological and molecular prevalence of lumpy skin disease virus in Korean water deer, native and dairy cattle in Korea. Korean Journal of Veterinary Service, 45(2), 133-137.
Matsiela M. S. et al. (2022). Improved safety profile of inactivated Neethling strain of the lumpy skin disease vaccine. Vaccine: X, 12, 100209.
Shumilova I. et al. (2022). A Recombinant Vaccine-like Strain of Lumpy Skin Disease Virus Causes Low-Level Infection of Cattle through Virus-Inoculated Feed. Pathogens, 11(8), 920.
Uzar S. et al. (2022). Comparison and efficacy of two different sheep pox vaccines prepared from the Bakırköy strain against lumpy skin disease in cattle. Clin Exp Vaccine Res; 11:1-11.
Adedeji A.J. et al. (2021) Household and animal factors associated with sheeppox and goatpox sero-prevalence and identification of high-risk areas in selected States of northern Nigeria. Preventive Veterinary Medicine,Volume 196,105473.
Ahmed E. M. et al. (2021). Lumpy skin disease outbreaks investigation in Egyptian cattle and buffaloes: Serological evidence and molecular characterization of genome termini. Comparative Immunology, Microbiology and Infectious Diseases, 76, 101639.
Fay P. et al. (2021). A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus. Transboundary and Emerging Diseases , 1-10.
Mansour M. E. et al. (2021). Sero Prevalence and Risk factors for Sheep Pox and Lumpy Skin Disease and Their Comparison to Capri Pox Double Antigen Multispecies ELISA in Khartoum and Kordofan States in Sudan. Archives of Clinical Microbiology, Vol.12 No.S3: 001.
Pandeya Y. et al. (2021). Case study of lumpy skin disease in cattle of Chitwan Nepal. National Cattle Research Program, Rampur, Nepal.
Sanz-Bernardo B. et al. (2021). Quantifying and modeling the acquisition and retention of lumpy skin disease virus by hematophagus insects reveals clinically but not subclinically affected cattleare promoters of viral transmission and key targets for control of disease outbreaks. J Virol 95:e02239-20.
Selim A. et al. (2021). Seroprevalence and risk factors for lumpy skin disease in cattle in Northern Egypt. Tropical Animal Health and Production, 53(3), 1-8.
Wolff J. et al. (2021). Development of a Safe and Highly Efficient Inactivated Vaccine Candidate against Lumpy Skin Disease Virus. Vaccines 9, 4
Kononov A. et al. (2020). Non-vector-borne transmission of lumpy skin disease virus. Scientific reports, 10(1), 1-12.
Krešić N. et al. (2020). Evaluation of serological tests for detection of antibodies against lumpy skin disease virus. Journal of Clinical Microbiology, 58(9)
Milovanović M. et al. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. Virology journal, 17(1), 1-7.
Wolff J. et al. (2020). Establishment of a Challenge Model for Sheeppox Virus Infection. Microorganisms, 8(12), 2001.
Wolff J. et al. (2020). Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia. Viruses, 12(7), 768.
Aldeewan, A. B. et al. (2019). Clinical and serological study of Lumpy skin disease in cattle in Basrah Provence. Kufa Journal For Veterinary Medical Sciences, 10(1).
Dawoud M. et al. (2019). Prevalence and molecular characterization of Lumpy Skin Disease in cattle during period 2016-2017. Benha Veterinary Medical Journal, 37(1), 172-175.
Milovanović M. et al. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. BMC Veterinary Research 15(1), 1-9
Möller J. et al. (2019). Experimental lumpy skin disease virus infection of cattle: Comparison of a field strain and a vaccine strain. Archives of virology, 164 (12), 2931-2941.
Ochwo S. et al. (2019). Seroprevalence and risk factors for lumpy skin disease virus seropositivity in cattle in Uganda. BMC Veterinary Research 15:236.
Samojlović M. et al. (2019). Detection of antibodies against lumpy skin disease virus by virus neutralization test and elisa methods. Acta Veterinaria-Beograd, 69 (1), 47-60.
Freeze-dried bovine serum negative for various diseases. Read more
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