Veterinary Diagnostics

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Lumpy Skin Disease Virus (LSDV) / Capripoxvirus (CPV)

ID Screen® Capripox Double Antigen Multi-species

ELISA

Double Antigen ELISA for the detection of antibodies against capripoxviruses including lumpy skin disease virus (LSDV), sheeppox virus (SPPV) and goatpox virus (GTPV) in serum or plasma from cattle, sheep, goats or other susceptible species.

Advantages

Specifications

Format

References

Downloads

  • First commercially-available ELISA which allows for the detection of Lumpy Skin Disease antibodies
  • Very high specificity in CPV-free regions (>99.7%). Should not cross-react with parapox viruses
  • Detects antibodies against LSDV either in vaccinated or infected animals. At least equivalent sensitivity compared to IPMA and improved sensitivity compared to VNT (detection of antibodies as of 20 dpv up until at least 7 months post-vaccination)
  • Easy to handle, with ready-to-use reagents, and allows for high throughput screening without requiring high level containment facilities

Method :

Double Antigen ELISA

Species :

Ruminants and other susceptible species

Specimens :

Serum and plasma

Coated antigen :

CPV purified antigen

Conjugate :

CPV purified antigen-HRP (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

CPVDA-2P 2 plates 192 12 x 8-well strips
CPVDA-5P 5 plates 480 12 x 8-well strips
  1. Hussien M.O. et al. (2022). Serological, virological and molecular diagnosis of an outbreak of lumpy skin disease among cattle in Butana area, Eastern Sudan. Veterinary Medicine and Science, 1–7.
  2. Uzar S. et al. (2022). Comparison and efficacy of two different sheep pox vaccines prepared from the Bakırköy strain against lumpy skin disease in cattle. Clin Exp Vaccine Res; 11:1-11.
  3. Ibrahim A.I. et al. (2022). Serodiagnosis of Lumpy Skin Disease Using Sheep Pox Virus Compared to a Commercial ELISA Kit. Journal of Applied Veterinary Sciences, 7(1), pp. 46-52.
  4. Adedeji A.J. et al. (2021) Household and animal factors associated with sheeppox and goatpox sero-prevalence and identification of high-risk areas in selected States of northern Nigeria. Preventive Veterinary Medicine,Volume 196,105473.
  5. Fay P. et al. (2021). A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus. Transboundary and Emerging Diseases , 1-10.
  6. Mansour M. E. et al. (2021). Sero Prevalence and Risk factors for Sheep Pox and Lumpy Skin Disease and Their Comparison to Capri Pox Double Antigen Multispecies ELISA in Khartoum and Kordofan States in Sudan. Archives of Clinical Microbiology, Vol.12 No.S3: 001.
  7. Sanz-Bernardo B. et al. (2021). Quantifying and modeling the acquisition and retention of lumpy skin disease virus by hematophagus insects reveals clinically but not subclinically affected cattleare promoters of viral transmission and key targets for control of disease outbreaks. J Virol 95:e02239-20.
  8. Selim A. et al. (2021). Seroprevalence and risk factors for lumpy skin disease in cattle in Northern Egypt. Tropical Animal Health and Production, 53(3), 1-8.
  9. Wolff J. et al. (2021). Development of a Safe and Highly Efficient Inactivated Vaccine Candidate against Lumpy Skin Disease Virus. Vaccines 9, 4
  10. Kononov A. et al. (2020). Non-vector-borne transmission of lumpy skin disease virus. Scientific reports, 10(1), 1-12.
  11. Krešić N. et al. (2020). Evaluation of serological tests for detection of antibodies against lumpy skin disease virus. Journal of Clinical Microbiology, 58(9)
  12. Milovanović M. et al. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. Virology journal, 17(1), 1-7.
  13. Milovanović M. et al.(2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. Virology journal, 17(1), 1-7.
  14. Wolff J. et al. (2020). Establishment of a Challenge Model for Sheeppox Virus Infection. Microorganisms, 8(12), 2001.
  15. Wolff J. et al. (2020). Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia. Viruses, 12(7), 768.
  16. Aldeewan, A. B. et al. (2019). Clinical and serological study of Lumpy skin disease in cattle in Basrah Provence. Kufa Journal For Veterinary Medical Sciences, 10(1).
  17. Dawou M. et al. (2019). Prevalence and molecular characterization of Lumpy Skin Disease in cattle during period 2016-2017. Benha Veterinary Medical Journal, 37(1), 172-175.
  18. Milovanović M. et al. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. BMC Veterinary Research 15(1), 1-9
  19. Möller J. et al. (2019). Experimental lumpy skin disease virus infection of cattle: Comparison of a field strain and a vaccine strain. Archives of virology, 164 (12), 2931-2941.
  20. Ochwo S. et al. (2019). Seroprevalence and risk factors for lumpy skin disease virus seropositivity in cattle in Uganda. BMC Veterinary Research 15:236.
  21. Samojlović M. et al. (2019). Detection of antibodies against lumpy skindisease virus by virus neutralization test and elisa methods. Acta Veterinaria-Beograd, 69 (1), 47-60.