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Foot and Mouth Disease Virus (FMDV)

ID Screen® FMD NSP Competition

ELISA

Competitive ELISA for the detection of Foot and Mouth Disease 3ABC non structural protein antibodies (NSP) in serum and plasma from bovine, ovine, caprine, porcine and all susceptible species

*Note: The ID Screen® product requires 4 control wells per run, and not 6 as with other commercial ELISAs. As a result, the maximum number of field samples which may be tested per plate is 92, and not 90.
A protocol including weak positive controls is available upon request. Please contact us for more information.

Advantages

Specifications

Format

References

Downloads

  • High sensitivity and specificity
  • Short and overnight incubations: possibility to deliver same-day FMD results when using the short protocol
  • Easy-to-use: no freeze-dried reagents and all dilution buffers are supplied coloured and ready-to-use
  • Efficiently detects carrier animals
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Method :

Competitive ELISA (solid phase blocking ELISA)

Species :

Ruminants, swine and all susceptible species

Specimens :

Serum and plasma

Coated antigen :

3ABC recombinant protein

Conjugate :

Anti-3ABC NSP-HRP (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

FMDNSPC-5P 5 plates 480 12 x 8-well strips
FMDNSPC-10P 10 plates 960 12 x 8-well strips

PERFORMANCE EVALUATIONS:

  • Keck H. et al. (2022). Proof of Proficiency of Decentralized Foot-and-Mouth Disease Virus Diagnostics in Germany. Viruses 2022, 14, 1098.
  • Tewari A. et al. (2021). Development and Validation of Confirmatory Foot-and-Mouth Disease Virus Antibody ELISAs to Identify Infected Animals in Vaccinated Populations. Viruses, 13, 914.
  • Browning C. et al. (2020). Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology. Journal of Veterinary Diagnostic Investigation 32.6 (2020): 933-937.
  • Comtet L. et al. (2019). Proven performance for FMDV NSP antibody detection with the ID Screen® FMD NSP Competitive ELISA. Poster presented at the GFRA Scientific Meeting, October 29-31, 2019, Bangkok, Thailand.
  • DEFRA EVID4 Evidence Project Final Report Pirbright Institute (2018). Development and evaluation of improved diagnostic tests for vesicular viral diseases of livestock.
  • Dill V. et al. (2017). Inter-laboratory validation of foot-and-mouth disease diagnostic capability in Germany. Veterinary Microbiology 203 62–67.

LARGE AND SMALL RUMINANTS:

  • Udahemuka J. C. et al. (2022). Investigation of foot and mouth disease virus and other animal pathogens in cattle, buffaloes and goats at the interface with Akagera National Park 2017–2020. BMC Veterinary Research, 18(1), 1-12.
  • Xaydalasouk K. et al. (2021). High seroprevalence of Foot and Mouth Disease in Laos: Call for nationwide vaccination campaigns and disease surveillance. Transboundary and Emerging Diseases, 68(4), 2345-2352.
  • Toklikishvili N. et al. (2020). Comparison Differentiation of Foot and Mouth Disease Virus Serotypes in Animals in High-Risk Zones of Georgia. J Anim Sci Res 5(1).
  • Hussain M. H. et al. (2019). Seroepidemiology of foot and mouth disease (FMD) virus non-structural protein (NSP) antibodies in the livestock of Oman. Acta tropica, 199, 105106.
  • Mesfine M. et al. (2019). Sero-Epidemiology of Foot and Mouth Disease in Domestic Ruminants in Amhara Region, Ethiopia. Front. Vet. Sci. 6:130.

SMALL RUMINANTS:

  • Siengsanan-Lamont J. et al. (2023). Risk mapping using serologic surveillance for selected One Health and transboundary diseases in Cambodian goats. PLoS Negl Trop Dis 17(4): e0011244.
  • Lysholm S. et al. (2022). Seroepidemiology of selected transboundary animal diseases in goats in Zambia. Preventive Veterinary Medicine, 206, 105708.
  • Chepkwony E.C. et al. (2021). Epidemiological study on foot-and-mouth disease in small ruminants: Sero-prevalence and risk factor assessment in Kenya. PLoS ONE 16(8): e0234286.
  • Hosny W.A. et al. (2020). Field serological investigation for peste des petits ruminants, foot-and-mouth disease, and bluetongue diseases in illegally introduced animals in Egypt. Veterinary World, 13(8): 1661-1666.
  • Jabary O.M. et al. (2020). Serodiagnosis of foot and mouth disease antibodies in sheep and goat sera by using NSP-cELISA in Garmian region, Kurdistan, Iraq. J. Anim. Health Prod. 8(2): 55-58.
  • Johansson E. (2020). Transboundary caprine diseases in Zambia. Degree Project within the Veterinary Medicine Programme, stud.epsilon.slu.se.
  • Mitternacht L. (2019). Seroprevalence of foot and mouth disease and peste des petits ruminants in small ruminants in Zambia on the border to Tanzania. Degree Project within the Veterinary Medicine Programme, stud.epsilon.slu.se.
  • Albayrak H. et al. (2018). Serological evidence of foot and-mouth disease virus infection in goat breeds in the Samsun province of Turkey. Journal of the Hellenic Veterinary Medical Society, 68(3), 327-330.
  • Torsson E. et al. (2017). Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania. Infection Ecology & Epidemiology, 7:1368336.
  • Päärni M. (2016). Seroprevalence of CCPP, FMDV, BTV and BVDV among small ruminants in Tanzania– A minor field study with focus on differential diagnoses of peste des petits ruminants virus. Degree Project in Veterinary Medicine, Clinical Sciences ISSN: 1652-8697 (p21,22,25,26).

LARGE RUMINANTS:

  • Tolawak D. et al. (2023). Seroprevalence, Serotype, and Associated Risk Factors of Foot and Mouth Disease in Selected Districts of East Wollega Zone, Ethiopia. Ethiopia (January 18, 2023).
  • Yirdaw B. et al. (2023). Seroprevalence, serotyping, and associated risk factors of Foot and mouth diseases in Bovine in Western Amhara regional state, North western Ethiopia. researchsquare.com.
  • Estevez Garcia A.I. et al. (2022). Outbreaks of Foot-and-Mouth Disease in Burundi, East Africa, in 2016, Caused
    by Different Serotypes. Viruses 2022,14, 1077.
  • Shurbe M. et al. (2022). Seroprevalence and associated risk factors for foot and mouth disease virus seropositivity in cattle in selected districts of Gamo zone, Southern Ethiopia. Front. Vet. Sci. 9:931643.
  • Siengsanan-Lamont J. et al. (2022). Abattoir-Based Serological Surveillance and Spatial Risk Analysis of Foot-and-Mouth Disease, Brucellosis, and Q Fever in Lao PDR Large Ruminants. Trop.Med. Infect. Dis. 2022, 7, 78.
  • Belayneh N. et al. (2020). Modeling the transmission dynamics of foot and mouth disease in Amhara region, Ethiopia. Preventive veterinary medicine, 181, 104673.
  • Salim S.A.S. et al. (2020). Prevalence and risk factors of foot and mouth disease virus in Nineveh province, Iraq. Adv. Anim. Vet. Sci. 8(1): 1-10.
  • Hemida M. et al. (2018). Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016. Veterinary Quarterly, 38:1, 88-98.
  • Sulayeman M. et al. (2018). Isolation, molecular characterization and sero prevalence study of foot-and-mouth disease virus circulating in Central Ethiopia. BMC Veterinary Research 14:110.
  • Babangida D. et al. (2017). Sero survey of foot and mouth disease virus infection in cattle crossing some major border states in Northwestern Nigeria.FOLIA VETERINARIA, 61, 3: 12—18.
  • Elnekave E. et al. (2016). The serological response against foot and mouth disease virus elicited by repeated vaccination of dairy cattle. Vaccine 08.054.

CATTLE AND PIGS:

  • Kong L. et al. (2023). Foot-and-mouth disease Non-Structural Protein seropositivity in cattle and pigs in Cambodia. researchsquare.com.
  • Siengsanan-Lamont J. et al. (2021). Seroepidemiology of Foot and Mouth Disease using passive surveillance techniques in selected provinces of Lao PDR. Tropical Animal Health and Production, 53, 1-10.

CAMELIDS:

  • Body M.H.H. et al. (2019). Foot and mouth disease virus: Serological study of dromedary camels in Oman. Rev Sci Tech, 38(3), 731-736.
  • Azizi M. et al. (2017). Serological Detection of Foot and Mouth Disease (FMD) in Camels of South of Iran (Fars, Bushehr and Hormozgan Province.    Applied Animal Science Research Journal No 24 pp: 29-34.

EMERGENCY SUPPLY:

Associated products

Internal reference material
ELISA

Freeze-dried porcine negative serum. Read more

Internal reference material
ELISA

Freeze-dried caprine serum containing anti-3ABC NSP antibodies (from a goat immunized with 3ABC recombinant protein). Read more