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Home   »   Products   »   Veterinary diagnostics   »     »   Foot and Mouth Disease Virus (FMDV)   »   ID Screen® FMD NSP Competition
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Foot and Mouth Disease Virus (FMDV)

ID Screen® FMD NSP Competition

ELISA

Competitive ELISA for the detection of Foot and Mouth Disease 3ABC non structural protein antibodies (NSP) in serum and plasma from bovine, ovine, caprine, porcine and all susceptible species

*Note: The ID Screen® product requires 4 control wells per run, and not 6 as with other commercial ELISAs. As a result, the maximum number of field samples which may be tested per plate is 92, and not 90.
A protocol including weak positive controls is available upon request. Please contact us for more information.

Advantages

Specifications

Format

References

Downloads

  • High sensitivity and specificity
  • Short and overnight incubations: possibility to deliver same-day FMD results when using the short protocol
  • Easy-to-use: no freeze-dried reagents and all dilution buffers are supplied coloured and ready-to-use
  • Efficiently detects carrier animals
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Method :

Competitive ELISA (solid phase blocking ELISA)

Species :

Ruminants, swine and all susceptible species

Specimens :

Serum and plasma

Coated antigen :

3ABC recombinant protein

Conjugate :

Anti-3ABC NSP-HRP (concentrated 10X)

Product reference

Kit format

Reactions

Plate format
FMDNSPC-5P 5 plates 480 12 x 8-well strips
FMDNSPC-10P 10 plates 960 12 x 8-well strips
  • OIE Terrestrial Manual 2012; 2.1.5; Foot and mouth disease: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.01.05_FMD.pdf

 

LARGE AND SMALL RUMINANTS:

  • Browning C. et al. (2020).Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus non structural protein serology. Journal of Veterinary Diagnostic Investigation 32.6 (2020): 933-937
  • Mesfine M. et al.(2019). Sero-Epidemiology of Foot and Mouth Disease in Domestic Ruminants in Amhara Region, Ethiopia. Front. Vet. Sci. 6:130

 

SMALL RUMINANTS:

  • Albayrak H. et al. (2018). Serological evidence of foot and-mouth disease virus infection in goat breeds in the Samsun province of Turkey. Journal of the Hellenic Veterinary Medical Society, 68(3), 327-330
  • Torsson E. et al. (2017). Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania. Infection Ecology & Epidemiology, 7:1368336
  • Päärni M. (2016). Seroprevalence of CCPP, FMDV, BTV and BVDV among small ruminants in Tanzania – A minor field study with focus on differential diagnoses of peste des petits ruminants virus. Degree Project in Veterinary Medicine, Clinical Sciences ISSN: 1652-8697 (p21,22,25,26)

 

LARGE RUMINANTS:

  • DEFRA EVID4 Evidence Project Final Report Pirbright Institute (2018). Development and evaluation of improved diagnostic tests for vesicular viral diseases of livestock
  • Hemida M. et al. (2018). Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016. Veterinary Quarterly, 38:1, 88-98
  • Sulayeman M. et al. (2018). Isolation, molecular characterization and sero prevalence study of foot-and-mouth disease virus circulating in Central Ethiopia. BMC Veterinary Research 14: 110
  • Babangida D. et al. (2017). Sero survey of foot and mouth disease virus infection in cattle crossing some major border states in Northwestern Nigeria. FOLIA VETERINARIA, 61, 3: 12—18
  • Dill V. et al. (2017). Interlaboratory validation of foot and mouth disease diagnostic capability in Germany. Veterinary Microbiology 203, 62-67
  • Elnekave E. et al. (2016). The serological response against foot and mouth disease virus elicited by repeated vaccination of dairy cattle. Vaccine 08.054
  • Tewari A. et al. (2016). Comparison of two commercial NSP antibody tests (PrioCHECK® and IDvet®FMDV NS ELISAs) to detect infection in vaccinated animals (DIVA). Poster presented at the EuFMD Meeting-Cascais Portugal, Oct 2016

 

OTHER SPECIES:

  • Azizi M. et al. (2017). Serological Detection of Foot and Mouth Disease (FMD) in Camels of South of Iran (Fars, Bushehr and Hormozgan Province). Applied Animal Science Research Journal No 24 pp: 29-34
Contact your local rep

Associated products

Internal reference material
Negative freeze-dried porcine serum
ELISA

Freeze-dried porcine negative serum. Read more

Internal reference material
FMD 3ABC NSP positive freeze-dried serum
ELISA

Freeze-dried caprine serum containing anti-3ABC NSP antibodies (from a goat immunized with 3ABC recombinant protein). Read more