Veterinary Diagnostics

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Influenza A

ID Screen® Influenza A Antibody Competition Multi-species

ELISA

Competitive ELISA kit for the detection of antibodies against the nucleoprotein of the Influenza A virus in multiple species

Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.

Advantages

Specifications

Format

References

Downloads

  • Accurate and reliable results
    • Highly sensitive and specific
    • Positive and negative reference sera available as internal controls
  • Improved workflow
    • Easy-to-use, with results in under 2 hours
    • Comprehensive data analysis with the ID Soft™program
  • Flexible
    • Convenient multi-species test applicable to birds, horses, swine. Consult us for use in any other species.
    • For avian species, use with egg yolk samples which are easier to collect and cause less stress to the layers

Method :

Competitive ELISA

Species :

Multiple species, including horses, birds, swine and others

Specimens :

Serum, plasma, egg yolk, porcine oral fluid

Coated antigen :

Influenza A nucleoprotein (NP)

Conjugate :

Anti-NP-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

FLUACA-2P 2 plates 192 12 x 8-well strips
FLUACA-5P 5 plates 480 12 x 8-well strips
FLUACA-10P 10 plates 960 12 x 8-well strips

Horses:

  1. Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/ j.vetmic. 2010.08.014.

Birds:

  1. Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.
  2. Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.
  3. Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009.
  4. Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.

Swine :

  1. Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74.
  2. Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.

Dogs:

  1. De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).

Associated products

Serum transport
ELISA
Internal reference material
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Ready to use chicken serum pool containing significant and known antibody level against Avian Influenza virus. Read more

Internal reference material
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Ready-to-use specific pathogen free (SPF) chicken serum pool. Does not contain any antibodies against any avian pathogen. Read more

Internal reference material
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Freeze-dried porcine negative serum. Read more