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Home   »   Products   »   Veterinary diagnostics   »  Ruminants   »   Mycobacterium avium subsp. paratuberculosis (Map)   »   ID Screen® Paratuberculosis Indirect
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Mycobacterium avium subsp. paratuberculosis (Map)

ID Screen® Paratuberculosis Indirect

ELISA

Indirect absorbed ELISA for the detection of anti- Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk

Screening and Confirmation formats available. In the confirmation format, each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).

Advantages

Specifications

Format

References

Downloads

  • Accuracy and reliability
    • Ideal for herd certification and disease eradication programs thanks to optimal specificity while maintaining high sensitivity, with clear separation of positive and negative results
    • Robust results thanks to consistent performance between batches
    • Widely used in European Map control programs
    • Freeze-dried internal reference material available for use an an internal control (sold separately: MRI-PARA; MRI-PARAMILK)
  • Flexibility
    • Test serum, plasma or milk
    • Screening and confirmation* formats available
  • Improved workflow
    • Automatable and rapid : results in 90 minutes.
    • Short and overnight protocols

 

 

Method :

Indirect absorbed ELISA

Species :

Ovine, caprine, bovine (cattle or buffalo)

Specimens :

Serum, plasma or bovine milk

Coated antigen :

Purified MAP extract

Conjugate :

Anti-ruminant-HRP conjugate (concentrated 10X)

Incubations :

Short or overnight

Screening Format

Product reference

Kit format

Reactions

Plate format
PARAS-5P 5 plates 480 12 x 8-well strips
PARAS-10P 10 plates 960 12 x 8-well strips

Confirmation Format

Product reference

Kit format

Reactions

Plate format
PARAB-5P 5 plates (biwell) 240 6 x 16-well strips

BOVINE

Cattle serum

  1. Caldow G. L. et al. (2023). Estimation of the specificity of an antibody ELISA for paratuberculosis generated from a sector of the UK cattle population using results from a paratuberculosis control program. Preventive Veterinary Medicine, 215, 105910.
  2. Corbiere F. et al. (2023). Effects of Silirum®-Based Vaccination Programs on Map Fecal Shedding and Serological Response in Seven French Dairy Herds. Animals, 13(9), 1569.
  3. Kolivand A. et al. (2023). Comparison to Methods; Serum Antibody ELISA and Fecal Nested-PCR to Diagnose Mycobacterium avium Subspecies Paratuberculosis Subspecies Infection in Cattle. Journal of Veterinary Research/Majallah-i Taḥqīqāt-i Dāmpizishkī University, 78(1).
  4. Echeverria G. et al. (2020). Prevalence of paratuberculosis in dairy cattle in Ecuador. The International Journal of Mycobacteriology, 9(1), 1-6.
  5. Sharma M. L. et al. (2019). Demonstration of Circulating Antibodies of Mycobacterium avium Subspecies paratuberculosis in Cattle of Rupandehi District, Nepal. Nepalese Veterinary Journal, 36, 23-29.
  6. Fox N. J. et al. (2018). Counterintuitive increase in observed prevalence in sympatric rabbits following the introduction of paratuberculosis control measures in cattle. Veterinary Record, 182(22), 634-634.
  7. Galiero A. et al. (2017). Serological survey of paratuberculosis in dairy cattle in Garfagnana district (Tuscany). Journal of the Hellenic Veterinary Medical Society, 68(4), 641-646.
  8. Shaughnessy R.G. et al. (2015). Analysis of Biobanked Serum from a Mycobacterium avium subsp paratuberculosis Bovine Infection Model Confirms the Remarkable Stability of Circulating miRNA Profiles and Defines a Bovine Serum miRNA Repertoire. PLoS ONE 10(12): e0145089.
  9. Brugel C. et al. (2014). Facing interpretation difficulties of positive serology in presupposed paratuberculosis-free herds: longitudinal study and result comparison of commercial ELISAs. Poster presented at the 12th ICP – International Colloquium on Paratuberculosis – Parma, Italy.
  10. Comtet L. et al. (2014). Use of the interferon-gamma release assay for the detection of Mycobacterium avium subsp. paratuberculosis infection. Poster presented at the 12th ICP – International Colloquium on Paratuberculosis – Parma, Italy – June 2014.
  11. Verité S. et al. (2014). Paratuberculosis ELISAs: improvements on the horizon? Poster presented at the 12th ICP – International Colloquium on Paratuberculosis – Parma, Italy – June 2014.
  12. Medeiros J.M.A. et al. (2012). Frequência de anticorpos para paratuberculose em bovinos no semiarido paraíbano. Pesq. Vet. Bras. 32(8):697-700. (in Portuguese).
  13. Pozzato N. et al. (2010). Evaluation of four commercial serum ELISA kits for the detection of bovine paratuberculosis: an interlaboratory and field trial. Poster at the Epizone Meeting 2010, Saint-Malo, France.
  14. Fry M. et al. (2008). Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332
  15. Köhler H. et al. (2008). Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210.

Cattle milk

  1. Kirkeby C. et al. (2016). Simulating the Epidemiological and Economic Impact of Paratuberculosis Control Actions in Dairy Cattle. Front. Vet. Sci. 3:90.
  2. Kirkeby C. et al. (2016). Adaptive Test Schemes for Control of Paratuberculosis in Dairy Cows. tPLoS ONE 11(12) e0167219.
  3. Lavers C. J. et al. (2014). Evaluation of milk ELISA for detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and association with within-herd prevalence. Journal of Dairy Science, 97(1), 299-309.
  4. Micheloud J.F. et al. (2014). Diagnóstico de paratuberculosis bovina en la cuenca lechera del valle de Lerma, Salta. XX Reunión científico Técnica. Tucumán, Argentina (in spanish).
  5. Nielsen S. et al. (2014). Bulk tank milk ELISA for detection of antibodies to Mycobacterium avium subsp. paratuberculosis: Correlation between repeated tests and within-herd antibody-prevalence. Preventive Veterinary Medicine. 11396-102.
  6. Nielsen S. et al. (2013). Dynamics of Specific Anti-Mycobacterium avium Subsp. paratuberculosis Antibody Response through Age. Plos One, Vol. 8, Issue 4, e63009.
  7. Zervens L. M.L. et al. (2013). Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum. The Veterinary Journal 197 889-891.
  8. Nielsen S. et al. (2012). Effect of days in milk and milk yield on testing positive in milk antibody ELISA to Mycobacterium avium subsp. paratuberculosis in dairy cattle. Veterinary Immunology and Immunopathology 149) 6-10.
  9. Thomsen V. et al. (2012). Characterization of the long-term immune response to vaccination against Mycobacterium avium subsp. paratuberculosis in Danish dairy cows. Veterinary Immunology and Immunopathology 145 316-322.

Cattle milk and serum

  1. Weber M. F. et al. (2022). Predicting Positive ELISA Results in Dairy Herds with a Preferred Status in a Paratuberculosis Control Program. Animals, 12(3), 384.
  2. Saad N.M. et al. (2021). Detection of Mycobacterium avium subsp. paratuberculosis in cow’s milk. Journal of Microbiology, Biotechnology and Food Sciences, 7(6).
  3. Kennedy A.E. et al. (2016). Analysis of Johne’s disease ELISA status and associated performance parameters in Irish dairy cows. BMC Veterinary Research12:43.
  4. McAloon C.G. et al. (2016). Bayesian estimation of prevalence of paratuberculosis in dairy herds enrolled in a voluntary Johne’s Disease Control Programme in Ireland. Preventive Veterinary Medicine 128 95–100.
  5. Kirkeby C. et al. (2015). Mean effective sensitivity for Mycobacterium avium subsp. paratuberculosis infection in cattle herds. BMC Veterinary Research 11:190.
  6. INSTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA LOMBARDIA E DELL’EMILIA ROMAGNA (2014). Procedura aperta telematica finalizzata alla conclusione di un accordo quadro in unione di acquisto per la fornitura di kit per ricerca Mycobacterium paratuberculosis. Fascicolo n.1101. (in italian?)
  7. Kennedy A. et al. (2014). The single intradermal cervical comparative test interferes with Johne’s disease ELISA diagnostics. Frontiers in Immunology. Vol. 5, Art. 564.

Buffaloe serum

  1. Martucciello A. et al. (2021). Seroprevalence of paratuberculosis in Italian water buffaloes (Bubalus bubalis) in the region of Campania. Journal of Dairy Science, 104(5), 6194-6199.
  2. Ricchi M. et al. (2018). Evaluation of serological test for the detection of paratuberculosis in Italian buffaloes (Bubalus bubalis): a class latent approach. Poster IPCV.
  3. Uy M.R. et al. (2018). Serological and molecular evaluation of Mycobacterium avium subspecies paratuberculosis (Johne’s disease) infecting riverine-type water buffaloes (Bubalus bubalis) in the Philippines. Comparative Immunology, Microbiology and Infectious Diseases 61 24-29.
  4. Desio G. et al. (2013). Estimated prevalence of Johne’s disease in herds of water buffaloes (Bubalus bubalis) in the province of Caserta. Italian Journal of Animal Science, Vol. 12, N°1.

Buffaloe milk

  1. Hafiz N.M. et al. (2016). Detection of Mycobacterium avium subsp. paratuberculosis in raw buffaloe’s milk. Inter J ChemTech Res, 9, 123-8

SMALL RUMINANTS

  1. Schrott J. et al. (2023). Mycobacterium avium subsp. paratuberculosis in Sheep and Goats in Austria: Seroprevalence, Risk Factors and Detection from Boot Swab Samples. Animals, 13(9), 1517.
  2. Arteche-Villasol N. et al. (2022). Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis. Frontiers in Veterinary Science, 8, 744568.
  3. Fernández M. et al. (2022). Effects of Paratuberculosis Vaccination at Different Ages in a Dairy Goat Herd: A 2-Year Follow-Up. Animals, 12(22), 3135.
  4. Yu Y. et al. (2022). Identification of Mycobacterium avium subspecies paratuberculosis in sheep farms in Bayannaoer, Inner Mongolia, China. BMC Veterinary Research, 18(1), 1-6.
  5. Shabana I.I. et al. . (2020). Sero-surveillance of Mycobacterium avium subspecies paratuberculosis infection in ruminants in Medina. Journal of Advanced Veterinary and Animal Research, 7(1), 69.
  6. Iarussi F. et al. (2019). Epidemiology and risk factors of Mycobacterium avium subspecies paratuberculosis in semi-extensive dairy sheep and goat farms of Apulia, southern Italy. Small Ruminant Research, 177, 89-96.
  7. Lievaart-Peterson K. et al. (2019). Mycobacterium avium subspecies paratuberculosis DNA and antibodies in dairy goat colostrum and milk. Veterinary Sciences, 6(4), 96.
  8. Ma J.G. et al. (2019). First report of bovine viral diarrhea virus and Mycobacterium avium subspecies paratuberculosis infection in Tibetan sheep (Ovis aries) in Tibetan Plateau, China. Tropical animal health and production, 51, 719-722.
  9. Cecchi F. et al. (2018). Preliminary association analysis of microsatellites and Mycobacterium avium subspecies paratuberculosis infection in the native Garfagnina goats. JOURNAL OF APPLIED ANIMAL RESEARCH, VOL. 46, NO. 1, 879–882.
  10. Galiero A. et al. (2017). Serological, culture and molecular survey of Mycobacterium avium paratuberculosis in a goat flock in Tuscany. Folia Microbiol DOI 10.1007/s12223-017-0518-7 # Institute of Microbiology, Academy of Sciences of the Czech Republic
  11. Mathevon Y. et al. (2017). Estimation of the sensitivity and specificity of two serum ELISAs and one fecal qPCR for diagnosis of paratuberculosis in sub-clinically infected young-adult French sheep using latent class Bayesian modeling. BMC Veterinary Research, 13, 1-11.
  12. Moioli B. et al. (2016). Genomic scan for identifying candidate genes for paratuberculosis resistance in sheep. Animal Production Science, 56, 1046–1055.
  13. Lafort M.P. (2015). La paratuberculose chez les ovins‎: effet de la vaccination sur la réponse sérologique et l’excrétion fécale de Mycobacterium avium subspecies paratuberculosis (Map) (Doctoral dissertation, in French).
  14. Pérez, V et al. (2015). Evaluation of the sensitivity of the ID Screen® Paratuberculosis Indirect ELISA in ovine sera. Poster presented at the 17th International Symposium of the WAVLD – World Association of Veterinary Laboratory Diagnosticians – Saskatoon, Canada– June 2015.
  15. Köhler et al. (2015). Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection. BMC Veterinary Research 11:74
  16. Bergmann A. et al. (2015). In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis. PLoS ONE 10(4): e0123980.
  17. Ahmed I.M. (2010). Serodiagnosis of Johne’s disease by indirect ELISA in ovine. Iraqi Journal of Veterinary Sciences, Vol. 24, N°1 (41-43).
  18. Foucras G. et al. (2008). Five-year longitudinal study of serum antibody by absorbed ELISA in an ovine flock endemically infected by Mycobacterium avium subsp. Paratuberculosis (MAP). Poster and Presentation at World Buiatrics Congress, Budapest.

BOVINE AND SMALL RUMINANTS

  1. Borujeni M.P. et al. (2021). Comparison of Mycobacterium avium subsp. paratuberculosis infection in cattle, sheep and goats in the Khuzestan Province of Iran: Results of a preliminary survey. Veterinary Medicine and Science, 7(5), 1970-1979.
  2. A. et al. (2013). Epidemiological studies on Johne’s disease in ruminants and Crohn’s disease in humans in Egypt. International Journal of Veterinary Science and Medicine. 1, (79-86).

OTHER SPECIES

  1. Pigoli C. et al. (2020). Paratuberculosis in Captive Scimitar-Horned Oryxes (Oryx dammah). Animals, 10(11), 1949.
  2. Salem M. A. et al. (2019). Investigation of Mycobacterium paratuberculosis in Arabian dromedary camels (Camelus dromedarius). Veterinary World, 12(2), 218.
  3. Stanitznig A. et al. (2017). Prevalence of Mycobacterium avium subspecies paratuberculosis and hepatitis E in New World camelids in Austria. Veterinary Record, 181(2), 46-46.
  4. Meng K.F. et al. (2015). Seroprevalence and risk factors of Mycobacterium avium subspecies paratuberculosis infection in domestic sika deer in China. Trop Anim Health Prod. 47, 999-1003.
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