Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis
This test does not contain any virus particles.
Equivalent performance to RT-PCR
Improved sensitivity for samples with weak or strong PPRV titres
Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells
Straightforward and rapid protocol, with results in 90 minutes
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Ovine and caprine
Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Coated antigen :
Anti-PPRV NP antibody
Anti-PPRV-NP Mab-HRP conjugate (concentrated 10X)
PPRAG-1P1 plate9612 x 8-well strips
PPRAG-2P2 plates19212 x 8-well strips
O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231
Recognizing peste des petits ruminants – A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR
Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304
Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108
Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT- PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335
Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant- diseases/serodiagnosis-of-peste-des-petits-ruminants-virus
Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.
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