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Peste des Petits Ruminants Virus (PPRV)
ID Screen® PPR Antigen Capture
ELISA 
- Equivalent performance to RT-PCR
- Improved sensitivity for samples with weak or strong PPRV titres
- Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
- Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells
- Straightforward and rapid protocol, with results in 90 minutes
- Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Method :
Sandwich ELISASpecies :
Ovine and caprineSpecimens :
Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMCCoated antigen :
Anti-PPRV NP antibodyConjugate :
Anti-PPRV-NP Mab-HRP conjugate (concentrated 10X)Product reference
Kit format
Reactions
Plate format
PPRAG-1P
1 plate
96
12 x 8-well strips
PPRAG-2P
2 plates
192
12 x 8-well strips
- O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231
- Recognizing peste des petits ruminants – A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR
- Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304
- Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108
- Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT- PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335
- Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant- diseases/serodiagnosis-of-peste-des-petits-ruminants-virus
- Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.
Associated products
ELISA