IgM Antibody Capture ELISA (MAC) kit for the detection of anti-prE IgM antibodies in horse serum and plasma.
The detection of IgM antibodies indicates recent infection and WNV circulation.
Advantages
Specifications
Format
References
Downloads
Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection
Rapid and convenient: all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Method :
IgM Antibody Capture ELISA
Species :
Equine
Specimens :
Serum and plasma
Coated antibody :
Anti-horse IgM polyclonal antibody
Conjugate :
Anti-WNV prE HRP conjugate (10X)
Protocol :
(1) Sample Incubation (2) Three washes (3) WNV ready-to-use antigen incubation (4) Anti-WNV prE-HRP conjugate incubation (5) Three washes (6) Substrate Incubation: 15 min
Product reference
Kit format
Reactions
Plate format
WNIGM-1P1 plate (biwell format)486 x 16-well strips
WNIGM-4P4 plates (biwell format)1926 x 16-well strips
Lourenço J. et al. (2022). West Nile virus transmission potential in Portugal. Communications Biology, 5(1), 1-12.
Scaramozzino P. et al. (2021). West Nile and Usutu viruses co-circulation in central Italy: Outcomes of the 2018 integrated surveillance. Parasites & Vectors, 14(1), 1-7.
Beck C. et al. (2020). Contrasted epidemiological patterns of West Nile virus lineages 1 and 2 infections in France from 2015 to 2019. Pathogens, 9(11), 908.
Folly A. J. et al. (2020). Equine seroprevalence of West Nile virus antibodies in the UK in 2019. Parasites & Vectors, 13(1), 1-5.
de Heus P. et al. (2020). Emergence of West Nile virus lineage 2 in Europe: Characteristics of the first seven cases of West Nile neuroinvasive disease in horses in Austria. Transboundary and emerging diseases, 67(3), 1189-1197.
Barros S. et al. (2017). West Nile virus in horses during the summer and autumn seasons of 2015 and 2016, Portugal. Veterinary Microbiology 212, 75–79.
Beck C. et al. (2017). Improved reliability of serological tools for the diagnosis of West Nile fever in horses within Europe. PLoS Negl Trop Dis11(9): e0005936.
Bahuon C. et al. (2016). West Nile virus epizootics in the Camargue (France) in 2015 and reinforcement of surveillance and control networks. Sci. Tech. Off. Int. Epiz., 35 (3), 811-824.
Monaco F. et al. (2015). The 2011 West Nile disease outbreak in Sardinia region, Italy. Veterinaria Italiana 2015, 51 (1), 5-16.
Barbić L. et al. (2013). West Nile virus serosurveillance in horses in Croatia during the 2012 transmission season. Medical Sciences, 39 (2013): 95-104.
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