IgM Antibody Capture ELISA (MAC) kit for the detection of anti-prE IgM antibodies in horse serum and plasma.
The detection of IgM antibodies indicates recent infection and WNV circulation.
Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection
Rapid and convenient: all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
IgM Antibody Capture ELISA
Serum and plasma
Coated antibody :
Anti-horse IgM polyclonal antibody
Anti-WNV prE HRP conjugate (10X)
(1) Sample Incubation (2) Three washes (3) WNV ready-to-use antigen incubation (4) Anti-WNV prE-HRP conjugate incubation (5) Three washes (6) Substrate Incubation: 15 min
WNIGM-1P1 plate (biwell format)486 x 16-well strips
WNIGM-4P4 plates (biwell format)1926 x 16-well strips
Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156
OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.