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West Nile Virus (WNV) & Flaviviruses

ID Screen® West Nile IgM Capture


IgM Antibody Capture ELISA (MAC) kit for the detection of anti-prE IgM antibodies in horse serum and plasma.

The detection of IgM antibodies indicates recent infection and WNV circulation.






  • Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection
  • Rapid and convenient: all reagents are supplied ready-to-use
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera

Method :

IgM Antibody Capture ELISA

Species :


Specimens :

Serum and plasma

Coated antibody :

Anti-horse IgM polyclonal antibody

Conjugate :

Anti-WNV prE HRP conjugate (10X)

Protocol :

(1) Sample Incubation (2) Three washes (3) WNV ready-to-use antigen incubation (4) Anti-WNV prE-HRP conjugate incubation (5) Three washes (6) Substrate Incubation: 15 min

Product reference

Kit format


Plate format

WNIGM-1P 1 plate (biwell format) 48 6 x 16-well strips
WNIGM-4P 4 plates (biwell format) 192 6 x 16-well strips
  1. Lourenço J. et al. (2022). West Nile virus transmission potential in Portugal. Communications Biology, 5(1), 1-12.
  2. Scaramozzino P. et al. (2021). West Nile and Usutu viruses co-circulation in central Italy: Outcomes of the 2018 integrated surveillance. Parasites & Vectors, 14(1), 1-7.
  3. Beck C. et al. (2020). Contrasted epidemiological patterns of West Nile virus lineages 1 and 2 infections in France from 2015 to 2019. Pathogens, 9(11), 908.
  4. Folly A. J. et al. (2020). Equine seroprevalence of West Nile virus antibodies in the UK in 2019. Parasites & Vectors, 13(1), 1-5.
  5. de Heus P. et al. (2020). Emergence of West Nile virus lineage 2 in Europe: Characteristics of the first seven cases of West Nile neuroinvasive disease in horses in Austria. Transboundary and emerging diseases, 67(3), 1189-1197.
  6. Barros S. et al. (2017). West Nile virus in horses during the summer and autumn seasons of 2015 and 2016, Portugal. Veterinary Microbiology 212, 75–79.
  7. Beck C. et al. (2017). Improved reliability of serological tools for the diagnosis of West Nile fever in horses within Europe. PLoS Negl Trop Dis11(9): e0005936.
  8. Bahuon C. et al. (2016). West Nile virus epizootics in the Camargue (France) in 2015 and reinforcement of surveillance and control networks. Sci. Tech. Off. Int. Epiz., 35 (3), 811-824.
  9. Monaco F. et al. (2015). The 2011 West Nile disease outbreak in Sardinia region, Italy. Veterinaria Italiana 2015, 51 (1), 5-16.
  10. Barbić L. et al. (2013). West Nile virus serosurveillance in horses in Croatia during the 2012 transmission season. Medical Sciences, 39 (2013): 95-104.

Associated products

Internal reference material