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Mycoplasma bovis

ID Screen® Mycoplasma bovis Indirect

ELISA

Indirect ELISA kit for the detection of antibodies against Mycoplasma bovis in bovine serum, plasma or milk

Advantages

Specifications

Format

References

Downloads

  • Excellent discriminatory capacity, giving clear positive or negative results
  • Excellent reproducibility, repeatability, stability and robustness
  • Excellent correlation of results between individual milk and serum samples, making the test versatile for testing beef cattle or milk samples from dairy herds
  • Superior specificity and sensitivity
  • Rapid protocol : results in 90 minutes
  • Direct dilution of samples in the plate, with ready-to-use sample buffer

Method :

Indirect ELISA

Species :

Cattle

Specimens :

Serum, plasma and milk

Coated antigen :

Microplates coated with purified Mycoplasma bovis recombinant antigen

Conjugate :

Anti-ruminant horseradish peroxidase (HRP) conjugate

Product reference

Kit format

Reactions

Plate format

MBOVISS-2P 2 plates 192 12 x 8-well strips
MBOVISS-5P 5 plates 480 12 x 8-well strips
MBOVISS-10P 10 plates 960 12 x 8-well strips
PERFORMANCE EVALUATION

MILK SAMPLES

  1. Bokma J. et al. (2024). Diagnostic performance of Mycoplasma bovis antibody ELISA tests on bulk tank milk from dairy herds. BMC Veterinary Research, 20(1), 81.
  2. McAloon C. I. et al. (2024). Estimation of sensitivity and specificity of bulk tank milk PCR and 2 antibody ELISA tests for herd-level diagnosis of Mycoplasma bovis infection using Bayesian latent class analysis. Journal of Dairy Science.
  3. Salgadu A. et al. (2023). Evaluating Cut-Offs for Use of the Id Screen Mycoplasma Bovis Elisa in Bulk Tank Milk in New Zealand. Available at SSRN 4614096.
  4. Lönsjö J. (2020). Evaluation of ELISA and qPCR assays for detection of Mycoplasma bovis in milk from ruminants. http://lup.lub.lu.se/student-papers/record/9020393

SERUM SAMPLES

  1. Marquetoux N. et al. (2023). Evaluation of the accuracy of the IDvet serological test for Mycoplasma bovis infection in cattle using latent class analysis of paired serum ELISA and quantitative real-time PCR on tonsillar swabs sampled at slaughter. PLoS ONE 18(5): e0285598.
  2. Veldhuis A. et al. (2023). Bayesian diagnostic test evaluation and true prevalence estimation of Mycoplasma bovis in dairy herds. Preventive Veterinary Medicine, 216, 105946.
  3. Andersson A. M. et al. (2019). A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis. BMC veterinary research, 15(1), 1-10.

MILK AND SERUM SAMPLES

  1. Petersen M. B. et al. (2020). Field experience of antibody testing against Mycoplasma bovis in adult cows in commercial Danish dairy cattle herds. Pathogens, 9 (8), 637.
EPIDEMIOLOGICAL STUDIES

MILK SAMPLES

  1. Hurri E. et al.  (2024). A longitudinal study of the dynamics of Mycoplasma bovis antibody status in primiparous cows and bulk tank milk in Swedish dairy herds. Journal of Dairy Science.
  2. Hayajneh F.M.F. et al. (2024). Epidemiological Investigations of Mycoplasma bovis-Associated Mastitis in Dairy Animals along with Analysis of Interleukin-6 (IL-6) as a Potential Diagnostic Marker. International Journal of Veterinary Science. Int J Vet Sci, 13(1), 120-126.
  3. Hurri E. et al. (2022). Herd-level prevalence of Mycoplasma bovis in Swedish dairy herds determined by antibody ELISA and PCR on bulk tank milk and herd characteristics associated with seropositivity. Journal of Dairy Science, 105(9), 7764-7772.
  4. McAloon C.I. et al. (2022). Seroprevalence of Mycoplasma bovis in bulk milk samples in Irish dairy herds and risk factors associated with herd seropositive status. Journal of Dairy Science, 105(6), 5410-5419.
  5. Mc Carthy M. C. et al. (2021). Longitudinal prevalence of antibodies to endemic pathogens in bulk tank milk samples from dairy herds engaged or not in contract heifer rearing. Frontiers in Veterinary Science, 1415.

SERUM SAMPLES

  1. Oucheriah Y. et al. (2022). Prevalence of Mycoplasma bovis in Algeria and Characterisation of the Isolated Clones. Frontiers in Veterinary Science, 9.

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