Veterinary Diagnostics

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Rift Valley Fever Virus (RVFV)

ID Screen® Rift Valley Fever IgM Capture

ELISA

IgM Antibody Capture (MAC) ELISA for the detection of anti-nucleoprotein IgM antibodies in ruminant serum and plasma

The detection of anti-IgM antibodies indicates recent infection.

This test does not contain any virus particles.

Advantages

Specifications

Format

References

Downloads

  • Accurate and reliable: excellent specificity and analytical and diagnostic sensitivity
  • Convenient formatready-to-use, coated plates in strips, and all other components in liquid format.

Method :

IMAC (IgM Antibody Capture) ELISA

Species :

Ruminants

Specimens :

Serum or plasma

Coated antigen :

RVF nucleoprotein (concentrated 10X)

Coated antibody :

Anti-bovine-ovine-caprine IgM antibody

Conjugate :

Anti-NP-HRP (concentré 10X)

Protocol :

1) Sample Incubation. 2) Three washes. 3) RVF nucleoprotein incubation. 4) Three washes. 5) Anti-NP-HRP conjugate incubation. 6) Three washes. 7) Substrate Incubation.

Product reference

Kit format

Reactions

Plate format

RIFTIGM-4P 4 plates (biwell format) 192 6 x 16 well strips
RIFTIGM-10P 10 plates (biwell format) 480 6 x 16 well strips
CATTLE
  • Stoek F. et al. (2022). Mosquito survey in Mauritania: Detection of Rift Valley fever virus and dengue virus and the determination of feeding patterns. PLoS Negl Trop Dis 16(4): e0010203.
  • Alhaji N. B. et al. (2020). Seropositivity and associated intrinsic and extrinsic factors for Rift Valley fever virus occurrence in pastoral herds of Nigeria: a cross sectional survey. BMC Veterinary Research, 16, 1-10.
  • Tshilenge G.M. et al. (2018). Seroprevalence and virus activity of Rift valley fever in cattle in eastern region of Democratic Republic of the Congo. Journal of veterinary medicine, 2018(1), 4956378.
  • Tshilenge G. et al. (2019). Seroprevalence of Rift Valley fever virus in cattle in the Democratic Republic of the Congo. Tropical animal health and production, 51, 537-543.
  • Matiko M.K. et al. (2018). Serological evidence of inter-epizootic/ interepidemic circulation of Rift Valley fever virus in domestic cattle in Kyela and Morogoro, Tanzania. PLoS Negl Trop Dis 12(11): e0006931.
SHEEP AND GOATS
  • Omani R. et al. (2024). Goat seropositivity as an indicator of Rift Valley fever (RVF) infection in human populations: A case-control study of the 2018 Rift Valley fever outbreak in Wajir County, Kenya. One Health, 19, 100921.
  • Muturi M. et al. (2023). Ecological and subject-level drivers of interepidemic Rift Valley fever virus exposure in humans and livestock in Northern Kenya. Scientific Reports, 13(1), 15342.
  • Cichon N. et al. (2021). Co-circulation of Orthobunyaviruses and Rift Valley Fever Virus in Mauritania, 2015. Front. Microbiol. 12:766977.
  • Tshilenge G.M. et al. (2019). Rift Valley fever virus in small ruminants in the Democratic Republic of the Congo. Onderstepoort Journal of Veterinary Research 86(1), a1737.
LARGE AND SMALL RUMINANTS
  • Johnson S A.M. et al. (2023). Evidence of Rift Valley Fever Virus Circulation in Livestock and Herders in Southern Ghana. Viruses, 15(6), 1346.
  • Barry Y. et al. (2022). Rift Valley fever, Mauritania, 2020: Lessons from a one health approach. One Health, 15, 100413.
  • Kainga H. et al. (2022). Seroprevalence and Associated Risk Factors of Rift Valley Fever in Livestock from Three Ecological Zones of Malawi. Pathogens, 11, 1349.
  • Sado F.Y. et al. (2022). Seroprevalence of Rift Valley fever virus in domestic ruminants of various origins in two markets of Yaounde, Cameroon. PLoS Negl Trop Dis 16(8): e0010683.
  • Wanjama J. et al. (2022). Sero-Epidemiological Survey of Rift Valley Fever Virus in Ruminants in Nyandarua County, Kenya. East African Agricultural and Forestry Journal, 86(1-2), 11-11.
  • Sindato C. et al. (2021). Safety, Immunogenicity and Antibody Persistence of Rift Valley Fever Virus Clone 13 Vaccine in Sheep, Goats and Cattle in Tanzania. Front. Vet. Sci. 8:779858.
  • Wekesa F.C. et al. (2021). Serological evidence of inter-epidemic circulation of Rift Valley fever virus in livestock in Kenya. East African Agricultural and Forestry Journal, 85(3 & 4), 13-13.
  • Zouaghi K. et al. (2021). First Serological Evidence of Crimean-Congo Hemorrhagic Fever Virus and Rift Valley Fever Virus in Ruminants in Tunisia. Pathogens 2021, 10, 769.
  • Opayele A.V. et al. (2019). Serological evidence of Rift Valley fever virus infection in slaughtered ruminants in Nigeria. Journal of Immunoassay and Immunochemistry, 40(4), 367-377.
  • Rissmann M. et al. (2017). Evidence for enzootic circulation of Rift Valley fever virus among livestock in Cameroon. Acta tropica, 172, 7-13.
  • Rissmann M. et al. (2017). Serological and genomic evidence of Rift Valley fever virus during inter-epidemic periods in Mauritania. Epidemiology and Infection, 145(5), 1058-1068.
WILDLIFE
  • Dondona A.C. et al. (2016). Rift Valley Fever Virus among Wild Ruminants, Etosha National Park, Namibia, 2011. Emerg Infect Dis. 2016;22(1):128-130.

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