IgM Antibody Capture (MAC) ELISA for the detection of anti-nucleoprotein IgM antibodies in ruminant serum and plasma
The detection of anti-IgM antibodies indicates recent infection.
This test does not contain any virus particles.
Advantages
Specifications
Format
References
Downloads
Accurate and reliable: excellent specificity and analytical and diagnostic sensitivity
Convenient format: ready-to-use, coated plates in strips, and all other components in liquid format.
Method :
IMAC (IgM Antibody Capture) ELISA
Species :
Ruminants
Specimens :
Serum or plasma
Coated antigen :
RVF nucleoprotein (concentrated 10X)
Coated antibody :
Anti-bovine-ovine-caprine IgM antibody
Conjugate :
Anti-NP-HRP (concentré 10X)
Protocol :
1) Sample Incubation. 2) Three washes. 3) RVF nucleoprotein incubation. 4) Three washes. 5) Anti-NP-HRP conjugate incubation. 6) Three washes. 7) Substrate Incubation.
Product reference
Kit format
Reactions
Plate format
RIFTIGM-4P4 plates (biwell format)1926 x 16 well strips
RIFTIGM-10P10 plates (biwell format)4806 x 16 well strips
CATTLE
Stoek F. et al. (2022). Mosquito survey in Mauritania: Detection of Rift Valley fever virus and dengue virus and the determination of feeding patterns. PLoS Negl Trop Dis 16(4): e0010203.
Alhaji N. B. et al. (2020). Seropositivity and associated intrinsic and extrinsic factors for Rift Valley fever virus occurrence in pastoral herds of Nigeria: a cross sectional survey. BMC Veterinary Research, 16, 1-10.
Tshilenge G.M. et al. (2018). Seroprevalence and virus activity of Rift valley fever in cattle in eastern region of Democratic Republic of the Congo. Journal of veterinary medicine, 2018(1), 4956378.
Tshilenge G. et al. (2019). Seroprevalence of Rift Valley fever virus in cattle in the Democratic Republic of the Congo. Tropical animal health and production, 51, 537-543.
Matiko M.K. et al. (2018). Serological evidence of inter-epizootic/ interepidemic circulation of Rift Valley fever virus in domestic cattle in Kyela and Morogoro, Tanzania. PLoS Negl Trop Dis 12(11): e0006931.
SHEEP AND GOATS
Omani R. et al. (2024). Goat seropositivity as an indicator of Rift Valley fever (RVF) infection in human populations: A case-control study of the 2018 Rift Valley fever outbreak in Wajir County, Kenya. One Health, 19, 100921.
Muturi M. et al. (2023). Ecological and subject-level drivers of interepidemic Rift Valley fever virus exposure in humans and livestock in Northern Kenya. Scientific Reports, 13(1), 15342.
Cichon N. et al. (2021). Co-circulation of Orthobunyaviruses and Rift Valley Fever Virus in Mauritania, 2015. Front. Microbiol. 12:766977.
Tshilenge G.M. et al. (2019). Rift Valley fever virus in small ruminants in the Democratic Republic of the Congo. Onderstepoort Journal of Veterinary Research 86(1), a1737.
LARGE AND SMALL RUMINANTS
Johnson S A.M. et al. (2023). Evidence of Rift Valley Fever Virus Circulation in Livestock and Herders in Southern Ghana. Viruses, 15(6), 1346.
Barry Y. et al. (2022). Rift Valley fever, Mauritania, 2020: Lessons from a one health approach. One Health, 15, 100413.
Kainga H. et al. (2022). Seroprevalence and Associated Risk Factors of Rift Valley Fever in Livestock from Three Ecological Zones of Malawi. Pathogens, 11, 1349.
Sado F.Y. et al. (2022). Seroprevalence of Rift Valley fever virus in domestic ruminants of various origins in two markets of Yaounde, Cameroon. PLoS Negl Trop Dis 16(8): e0010683.
Wanjama J. et al. (2022). Sero-Epidemiological Survey of Rift Valley Fever Virus in Ruminants in Nyandarua County, Kenya. East African Agricultural and Forestry Journal, 86(1-2), 11-11.
Sindato C. et al. (2021). Safety, Immunogenicity and Antibody Persistence of Rift Valley Fever Virus Clone 13 Vaccine in Sheep, Goats and Cattle in Tanzania. Front. Vet. Sci. 8:779858.
Wekesa F.C. et al. (2021). Serological evidence of inter-epidemic circulation of Rift Valley fever virus in livestock in Kenya. East African Agricultural and Forestry Journal, 85(3 & 4), 13-13.
Zouaghi K. et al. (2021). First Serological Evidence of Crimean-Congo Hemorrhagic Fever Virus and Rift Valley Fever Virus in Ruminants in Tunisia. Pathogens 2021, 10, 769.
Opayele A.V. et al. (2019). Serological evidence of Rift Valley fever virus infection in slaughtered ruminants in Nigeria. Journal of Immunoassay and Immunochemistry, 40(4), 367-377.
Rissmann M. et al. (2017). Evidence for enzootic circulation of Rift Valley fever virus among livestock in Cameroon. Acta tropica, 172, 7-13.
Rissmann M. et al. (2017). Serological and genomic evidence of Rift Valley fever virus during inter-epidemic periods in Mauritania. Epidemiology and Infection, 145(5), 1058-1068.
WILDLIFE
Dondona A.C. et al. (2016). Rift Valley Fever Virus among Wild Ruminants, Etosha National Park, Namibia, 2011. Emerg Infect Dis. 2016;22(1):128-130.
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