Mycobacterium bovis complex / Tuberculosis

ID Screen® Ruminant IFN-g

ELISA

Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g) in activated plasma or culture supernatant

Advantages

Specifications

Format

References

Downloads

Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample
Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol)
Practical: the standard protocol uses only 10μl per sample, meaning that less sample volume is required for cell activation

Method :

Sandwich ELISA

Species :

Bovine, ovine and caprine

Specimens :

Activated plasma or culture supernatant

Coated antigen :

Anti-ruminant IFN-g monoclonal antibody

Conjugate :

Anti-ruminant IFN-g HRP Mab conjugate

Product reference

Kit format

Reactions

Plate format

IFNG-2P 2 plates 192 12 x 8-well strips

IFNG-4P 4 plates 384 12 x 8-well strips

IFNG-10P 10 plates 960 12 x 8-well strips

Gomez-Buendia A. et al. (2022). IFN-Y assay for granting and maintenance of officially tuberculosis-free herd status and movement of cattle within the EU. Presented by Visavet, European Union Reference Laboratory for bovine tuberculosis at the Seventh International Conference on Mycobacterium bovis, 7th – 10th June 2022, Galway, Ireland
Mohammed F. et al. (2021). Detection of Mycobacterium bovis infection in goats and sheep slaughtered at four abattoirs in Jigawa state, Nigeria. West african Journal of Microbiology (2955-1633), 1(01), 69-77.
Kang S. S.et al. (2020). Evaluation of the Performance of Interferon Gamma Assays for the Diagnosis of Bovine Tuberculosis in Hanwoo beef cattle in Korea. SSRG International Journal of Veterinary Science – Volume 6 Issue 1
DGAL (2019). Tuberculose bovine : dispositions techniques au dépistage sur animaux vivants. DGAL/SDSPA/2019-581.
Arrieta-Villegas C. et al. (2018). Efficacy of parenteral vaccination against tuberculosis with heat-inactivated Mycobacterium bovis in experimentally challenged goats. PloS one, 13(5), e0196948.
Serrano M. et al (2018). Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis. Veterinary research, 49(1), 1-10.
Balseiro A. et al. (2017). Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep. PLoS One, 12(7), e0180546.
Schiavo L. et al. (2013). Comparison between Two Gamma-Ifn Assays and Intradermal Tuberculin Test for the Diagnosis of Tuberculosis in Water Buffalo (Bubalus Bubalis). Editorial Board, 1071.
Eraghi V. et al. (2019). Recombinant fusion protein of Heparin-Binding Hemagglutinin Adhesin and Fibronectin Attachment Protein (rHBHA-FAP) of Mycobacterium avium subsp. paratuberculosis elicits a strong gamma interferon response in peripheral blood mononuclear cell culture. Gut Pathogens, 11(1), 1-8.
Bach H. et al. (2018). Protein kinase G induces an immune response in cows exposed to Mycobacterium avium subsp. paratuberculosis. BioMed research international, 2018.
Fay P.C. et al. (2022). The immune response to lumpy skin disease virus in cattle is influenced by inoculation route. Front. Immunol. 13:1051008.
Abdelwahab M.G. et al.(2016). Evaluation of humoral and cell-mediated immunity of Lumpy Skin Disease vaccine prepared from local strainin calves and its related to maternal immunity. Journal of American Science, 21(10).
Khafagy H. et al. (2016). Preparation and field evaluation of live attenuated sheep pox vaccine for protection of calves against Lumpy Skin Disease. Benha Veterinary Medical Journal, 31(2), 1-7.
Dudek K. et al. (2018). Saponin-based vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves. Journal of veterinary research, 62(3), 269-273.
Pereyra R. et al. (2019). Evidence of reduced vertical transmission of Neospora caninum associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition. Veterinary immunology and immunopathology, 208, 53-57.
Anderson J.et al. (2013). Evaluation of the immunogenicity of an experimental subunit vaccine that allows differentiation between infected and vaccinated animals against Bluetongue Virus serotype 8 in cattle. Clinical and Vaccine Immunology, 20(8), 1115-1122.
Laurin E. L. et al. (2015). Novel cell preservation technique to extend bovine in vitro white blood cell viability. Plos one, 10(10), e0140046.
Tutorial video for IFNg testing produced by the European Reference Laboratory for Bovine Tuberculosis, VISAVET