Veterinary Diagnostics

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Mycobacterium bovis complex / Tuberculosis

ID Screen® Ruminant IFN-g

ELISA

Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g) in activated plasma or culture supernatant

Advantages

Specifications

Format

References

Downloads

  • Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample
  • Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol)
  • Practical: the standard protocol uses only 10μl per sample, meaning that less sample volume is required for cell activation

Method :

Sandwich ELISA

Species :

Bovine, ovine and caprine

Specimens :

Activated plasma or culture supernatant

Coated antigen :

Anti-ruminant IFN-g monoclonal antibody

Conjugate :

Anti-ruminant IFN-g HRP Mab conjugate

Product reference

Kit format

Reactions

Plate format

IFNG-2P 2 plates 192 12 x 8-well strips
IFNG-4P 4 plates 384 12 x 8-well strips
IFNG-10P 10 plates 960 12 x 8-well strips
  1. Comtet, L., Pourquier, P. (IDvet). ID Screen® Interferon gamma ELISA: improvement of analytical sensitivity and introduction of a standard reference control to improve result interpretation. Poster presented at the 2012 International Colloquium on Paratuberculosis, Sydney, Australia.

2. Tutorial video for IFNg testing produced by the European Reference Laboratory for Bovine Tuberculosis, VISAVET

Associated products

Internal reference material
ELISA

Positive freeze-dried bovine activated plasma. Read more

Internal reference material
ELISA

Positive freeze-dried ovine activated plasma. Read more

Internal reference material
ELISA

Positive freeze-dried caprine activated plasma. Read more

Internal reference material
ELISA

Freeze-dried bovine plasma with no interferon gamma. Read more

Internal reference material
ELISA

Positive freeze-dried buffalo activated plasma. Read more